Hi,

I have an experimental setup where I had to make three cDNA plates
to fit all my samples in (duplicate), all have a 5-point dilution
curve (triplicate) of the same sample.
When I try to analyze my data in qBase I run into problems, which
seem to me to be linked to the fact that there are three standard
curves for each gene, so the program will not calculate the
amplification efficiency for each gene.

(I have calculated the amlpification efficiency for each plate in
the LightCycler 480 software when I ran each of the plates, and it
is not necessarily the same for the same gene in separate runs, so
it would make more sense to include one amplification efficiency per
plate rather than one per gene.)

Has anyone had similar problems or have any ideas as to how I can
fix or get around this?

I will be very happy for any help/response!