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RNA quality/low expression of genes   Message List  
Reply | Forward Message #328 of 344 |
Re: [qBase] RNA quality/low expression of genes

Hi there
 
I have used geNORM to rank the genes I analyse and got good results.
later I have analysed more genes on the same samples and I have introduced the new data in the geNROM analysis...the ranking has changed completely ...genes that were stable before show instability and the oposite. I have attached a file with the two graphs ilustrating this. Please advice.

Regards,
Adrian

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Wed Nov 21, 2007 5:14 pm

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Hi, I have 2 questions: 1. I have samples with different quality I want to compare. I used 2 ref genes that are normally very stable in this tissue (we tested... 		isabelle.schrauwen
isabelle.sch...
Offline Send Email 		Oct 16, 2007
1:01 pm

Comments on question 1 Although you may have validated your reference genes before it is still advisible to verify the stability of these reference genes in ...
hellemans_jan
Offline Send Email 		Nov 19, 2007
10:36 am

Hi there I have used geNORM to rank the genes I analyse and got good results. later I have analysed more genes on the same samples and I have introduced the...
Adrian Marius Peres B...
aperesbota
Offline Send Email 		Dec 13, 2007
1:17 pm
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