Hello Marcelo,

qBase does not do any statistical analysis on biological replicates.
The program treats samples with the same name as technical replicates.
Samples with different names are considered different samples,
without recognizing biological replicates. Further statistical
analysis can be performed on the exported normalized relative quantities.

Jan

--- In qBase@yahoogroups.com, "Marcelo Laia" <marcelolaia@...> wrote:
>
> Hi,
>
> We did a analysis on qBASE and we found a problem in set up the proper
> biological replication.
>
> We have four biological replicates for each condition:
>
> condition 1 --> 24_1, 24_2, 24_3 and 24_4
> condition 2 --> 3dai_1, 3dai_2, 3dai_3 and 3dai_4
>
> After the analysis we choose the endogenous control genes and do the
> final step in qBASE.
>
> When we go to Results, Multi Gene view, the graphics shows all samples
> (24_1, 24_2, 24_3, 24_4, 3dai_1, 3dai_2, 3dai_3 and 3dai_4) instead of
> the two conditions.
>
> For jump this problem we renamed the samples of condition 1 to 24h and
> the samples for condition 2 to 3dai. Is this correct?
>
> After that change all expression to 24h are setted to 1 and the
> expressions for 3dai are variable. Is this correct?
>
> How I could test if the difference between conditions are statistical
> significantly? How test you advice me for to do this analysis? qBASE
> do this test?
>
> Thank you very much
>
> Marcelo
>