Hello. I'm new to qbase and I am having troubling determining the NF for my data. I am working with mice and using three reference genes, run three times in...
Hi, could you please provide me with a full reference for the software for my poseter presentation to be presented at ATS 2006 (American Thoracic Society)....
Dear Ben, First of all, NFs (normalization factors) are not ratios, but geometric averages of the reference gene expression levels. Secondly, they should be...
Hi Jo, Thanx for your advice concerning the inter-run callibrators. I used 3 samples for that, but now I'am running out of cDNA for these 3 samples. I made new...
Dear Janneke I assume you want to measure more samples and compare these with previously measured samples. Just make new cDNA of the inter-run calibrators,...
Beste Jo, Na je antwoord op mijn vorige bericht heb ik nog 2 vragen. In het Nederlands kan ik mijn "probleem" duidelijker uitleggen. Omdat ik 3 samples als...
Hello everyone, and thanks again for all the help with my last question. I was wondering if any one could help me with some new questions I had about qbase. ...
Hi, I'm having problems with the calculation-part of the qBase tool. I have data for 4 genes (53 samples each) entered. There are three IRCs (inter run...
Dear Claudius, Please send the qBase export of your experiment (jvdesomp @ gmail . com), with indication in the mail of which reference genes you want to use....
Hi again, Sorry for asking a dumb question - but what do the results mean that I get for the selected reference genes? If I select 3 reference genes out of 4...
... Indeed! All genes (both genes of interest and reference genes) are normalized, meaning that their expression level is divided by the normalization factor...
Hi - yet again... Sorry to bug again - but it looks like non of the "rescaling options" for the "Single Gene Histogram" seems to work very well: Selecting one...
I have some samples "deleted" in the raw data set - but in the main screen I don't exclude any of them - except H2O of course, which is excluded by default....
Okay - it seems that everything works fine if you enter the data for your genes and then do the calculation. But if, after everything is calculated and the...
Okay - it seems that everything works fine if you enter the data for your genes and then do the calculation. But if, after everything is calculated and the...
I have a question concerning qBase. I have annotated a project and in this project I have 4 different experiments (these experiments are biological replicates...
Hi there, I have a huge dataset 19 genes, 66 samples measured duplicated and when I tried to analyze my data in qBase and I encounterd some problems: 1. I want...
Statistical analysis of biological replicates was planned for the project part of qBase, but has not yet been implemented. Jan ... From: qBase@yahoogroups.com...
-Hi there, I have a question/remark regarding my previous questions. When I do the calculation with less genes, by example 3 reference genes and 2 genes of...
Hi there, I'am sorry for annoying you again. But again a question from me. I started with data-analysis in qBase, with just 2 genes of interest as I mentioned...
I think I may have found the problem. It appears that only if I do an inter-run calibration the relative quantities don't have a fixed "1" or "100%". If I...
Did you use the exact same sample for each run/gene? I mean, lets say you use sampleID:35 (whatever that may be in your case) for your IRC. Did you use the...
Hi Jo, Thanx for your advice concerning the inter-run callibrators. I used 3 samples for that, but now I'am running out of cDNA for these 3 samples. I made new...
Well Claudius, I used 3 the same samples as IRC's for all genes and they have the same name/number in run A and run B per gene. So that is 1 strange thing. ...
This may be a stupid question - but running on an iCycler I had the habit of just letting the software select the best threshold for me for each run. Now that...
Is the calibration with NRQs still insensitive to threshold settings if the reference gene and gene of interest were run on different plates as well? So if I...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
