Hello, I wanted to check in with the users group to make sure I'm on the right track. I have been entering data from a recent SYBR green experiment into qBase...
Dear Doris, Your experimental setup and data seems almost perfect! The range in NF is only 2.5 fold, which is really low (and hence good). Good luck with your...
Hello, I am using qBase version 1.3.4 Have had success with the program but continue to have two issues. One is an error message "Application -defined ...
Ik had een vraagje ivm het weergeven van de waarden van qBase in publicaties? Is het volgens jou voldoende om de CV's van de huishoudgenen weer te geven (om de...
... program but ... -defined ... to the ... problem ... This known bug is harmless. We are stil looking for an adequate solution. ... export ... have ... there...
I have a question concerning the use of the M-values in publications; Should this value only be mentioned when a number (e.g., 10) of housekeeping genes are...
Dear Charlotte We propose to mention both the CV of normalized expression levels, as well as the M factor (which is calculated on raw/not-yet-normalized ...
Hi Thankyou Jo for your response to my normalising question. I now have three housekeeping genes and two target genes with 4 qPCR runs/gene. Before I had a...
Hi I too am experiencing the runtime error 9 outlined by Claudius on May 12th when trying to expand an already calculated experiment. It seems to run into...
Hi I just downloaded qBase earlier this week, but I am having trouble getting the program started. The Excel program freezes everytime I try to analyze an...
Hi Alessandra I experienced the same program when trying to run qBase with excel 2000. Upgrading to excel 2003 fixed this problem straight away. Hope this...
Hi, Can anyone help me with the following. When I start qbase the following run time error 438 appears - object doesnt support this property or method. Anyone...
Hello, I have a question on data analysis that I am hoping the users of qBASE can help me with. I have performed a series of sybr green qpcr experiments and ...
A few things to think about: What stats testing was done on the microarray data that showed significance ie) were multiple sample corrections applied and FDR ...
I am new with qBase and think that is a good solution for my analysis. But I have a problem with my data. I have used three replicated in separated runs with a...
I think you should try out REST for this anlysis, it can do what your looking for pretty well. You can download it for free. Stephen ... qBASE can ... ...
Thanks for the suggestion. REST did indeed find significant changes in my timepoint data. I'm puzzled though as to the best use of qBASE since REST did not...
Hi everybody, I'm a new user of qBase and I need your experience. I don't really understand the significance of the Normalisation Factor (NF). In my sample set...
Hi All, I was also thinking about this NF value, I have used four reference genes and have huge difference in NF values. But I know that my RNA quantities at...
... YES! I was working more on M value, which I ... You are right. ... Please see acceptable M values (e.g. best 3 reference genes) in our original paper in...
... This is only true if you started with equal quantity/quality of input material. Inspecting NF histograms can be a quality control. If you started with...
Hi. Starting the qbase.xls in windows XP with excel 2000 installed displayes the dummy project and examples. Selecting an experiment and pressing analyze, a...
Hi Jacob I used to have this exact same problem, until I upgraded my Office package to get Excell 2003, then all bugs disappeared. I guess qBase doesn't run...
Hi Alex. Have you by any chance installed a java program overwriting any VBA modules, which is fixed by the new excel 2003 installation? ... Jacob Hofman-Bang ...
Hi! Does anyone have experience of running expression analyses with low- density array cards from ABI? If so, is an inter-run calibrator needed also for LDA...
Hi, Indeed, ABI tells customers that inter-run calibration is not required. We are, however, not aware of any proof from ABI that the run-to-run variation can...
Thank you for your quick reply! It seems to me that it may be worth while using an IRC after all... Kind regards, Anna Anna Witasp, PhD student Karolinska...
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