Hi. Starting the qbase.xls in windows XP with excel 2000 installed displayes the dummy project and examples. Selecting an experiment and pressing analyze, a...
Hi Jacob I used to have this exact same problem, until I upgraded my Office package to get Excell 2003, then all bugs disappeared. I guess qBase doesn't run...
Hi Alex. Have you by any chance installed a java program overwriting any VBA modules, which is fixed by the new excel 2003 installation? ... Jacob Hofman-Bang ...
Hi! Does anyone have experience of running expression analyses with low- density array cards from ABI? If so, is an inter-run calibrator needed also for LDA...
Hi, Indeed, ABI tells customers that inter-run calibration is not required. We are, however, not aware of any proof from ABI that the run-to-run variation can...
Thank you for your quick reply! It seems to me that it may be worth while using an IRC after all... Kind regards, Anna Anna Witasp, PhD student Karolinska...
Hello Charlotte, You can choose to display the error in the "Option" dialog (upper right corner). Cheers, Mike ... -- Dr. Michael Walter The Microarray...
Hi All, i do not use the qBase software in my RT work but use its principle to calculate the inter-run variation. I used to have number of samples allowing me...
Hi, I´m experiencing troubles when importing my relative quantification data from an ABI 7900 HT into qBase. The Excel sheets contain the required columns...
Hi, There may a problem with data files consisting of several data blocks as can be the case for ABI7900. You could try to remove all the lines between the ...
Hi again, I´m not sure I understand what you mean with data block and lines... I import one plate (LDA, 384 well) at a time, and each plate is an Excel- sheet...
Hi, I will upload a file to make the data blocks and removal of intervening lines more clear. If this does not solve your problem, please send your file to...
... really ... my ... quantity ... normalisation with ... red in ... input ... you ... values for ... expression. ... have a ... of ... to the ... by ... thank...
Hi, I've just got qBase and i'm trynig to start using it. I've imported my first runs, which are standard curves to test the efficiency of my primers. When I...
I am getting my first experience with relative quantification. My ABI system is just offering a relative quantification software but it only works when...
... Dear Jo at first: thanks a lot for your quick reply! I just want to tell you something about my samples(wheat samples from the field infected with the...
Dear Martina, qBase can be downloaded from http://medgen.ugent/qbase We experienced some mailserver issues, but these are solved now. Do not hesitate to get...
hi there I am trying to use qBASE but 've got some problems! I downloaded succesfully the zip and started to use it but only few options are available when I...
Dear Adrian You probably mean that only a few of the items in the work flow are available. That is perfectly normal. Some require manual interaction, and can...
Hi Jo I am doing expression profile and when I am making my standard curve I am using 1x, 1/2, 1/4, 1/8, 1/16 dilution series since my cDNA amount is limiting....
Hi, I have been running real time reactions on ABI-7300. For one particular sample I had 2 replicates and ran both the replicates in duplicates. But both the...
Hi All, Just wondering is there a difference between the way GeNorm caluclates M value in comparison to Qbase. When I calculate with GeNorm I get M values of...
Dear Stephen Both qBase and geNorm use the same formula to calculate the M value. You do realize that the input is different for geNorm (should be relative ...
please note that we are talking about relative quantification, so the units are arbitrary units or 'fold change compared to the calibrator sample', or if you...
Dear Jo, Yes, my input for geNorm was the relative quantities, I divided the lowest CT value by every other sample. I have also double checked that its the...
Dear Stephen I'm afraid your calculations are not correct. You are not supposed to divide Ct values. Please take a look at the example calculation file on the...
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