qBase : Messages : 17-46 of 344

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17	Hello everybody When I try to import a csv result run, I have a message 'error 1004'. I use an ABI Prism 7000. Otherwise, my export table contain 'Standard...	denisplatel	Jul 7, 2005 8:59 am
18	hello, when I want to import my exel folder I met an error 1004...and it says me that an error occur during the columns rearengement... I work with the IQ...	damienpalomo	Jul 8, 2005 6:36 am
19	Whenever errors occur during import take a careful look at the requirements for the input files in the manual. The column headers have to be exactly as stated...	hellemans_jan	Jul 8, 2005 6:44 am
20	qBase sounds like a fabulous way to manage real time data but I simply cant get it to work. Firstly when I import my data from a Rotergene 3000 data file I...	e_cattell	Jul 12, 2005 3:45 pm
21	Dear, We are aware of the fact that importing data is sometimes troublesome. This is mainly because we did not have the software from every manufacturer...	Jan Hellemans hellemans_jan	Jul 12, 2005 7:49 pm
22	Okey, i am confused about this matter. I have two different samples. One is 5 untreated/control samples (same kind) and the other is a 50 treated samples (same...	privsky	Jul 18, 2005 6:14 am
23	hello, I just download the qBase software but unfortunately I do not understand well the operation of the software... is there French among the members of this...	laupreisser	Jul 20, 2005 1:37 pm
24	You could use the non-parametric equivalent of the t-test, e.g. the Mann-Whitney U, or a randomization test. Jo...	jvdesomp	Jul 28, 2005 7:58 am
25	Hi, First let me say that qbase looks like one of the most user friendly programmes available for rtPCR data handling I have come across. Thank you for writing...	Quentin Anstee drq92002	Aug 8, 2005 7:53 am
26	Dear, after reading the posted messages I could recreate all of the problems posted with importing data. I use a MyIQ-BioRad machine and my output files look...	kolipidos	Aug 8, 2005 7:55 am
27	Dear Dr. A. Schiel The manual/previously-messege-replies states that the import file should look EXACTLY like it does in the manual.. I've discovered that the...	neuronorm	Aug 8, 2005 12:45 pm
28	Hello, I use a MyIQ software and I try to use qBase but with no success. The VHL example doesn't work: I can't analyse the VHL example. When I try to do it,...	f_bernh	Aug 18, 2005 7:02 am
29	Some users experience severe problems with qBase, and are not even able to analyze the VHL experiment example. This is a known bug of qBase which occurs only...	hellemans_jan	Aug 23, 2005 1:38 pm
30	We just released a new version of qBase with new features and bug fixes, and an updated manual. We recommend to install the new version by going to the qBase...	Jo Vandesompele jvdesomp	Aug 27, 2005 4:37 pm
31	Dear Jo and Jan, I want to compare qBASE to the Genex Macro from Bio-Rad. I have a problem to get qBase to analyse. We want to analyse the expression of 4...	mvlig	Aug 29, 2005 10:31 am
32	Dear Marcel, You're on the right track! Your experimental design is perfect (each gene on another plate). If all goes well, qBase should provide you with ...	jvdesomp	Aug 29, 2005 2:52 pm
33	Hello, I tried to install the qbase software on my PC (Windows 98). I wasn't able to 'view certificate' and to install the digital certificate. I enabled all...	charlotjedhulst	Aug 30, 2005 9:46 am
34	... The digital certificate is made to avoid working on an unsecure level (allow all macros) or to allow the qBase macros (medium security level) every time...	hellemans_jan	Aug 30, 2005 9:51 am
35	... wasn't ... certificate. I ... qBase. ... level ... still ... I'm using office 2000. But I'm planning to install XP....	charlotjedhulst	Aug 30, 2005 12:35 pm
36	I now installed windows XP with office 2003. I still get the same error 1004? Charlotte...	charlotjedhulst	Aug 31, 2005 9:42 am
37	Hi, I would like to know if section 5 (Avaible imput formats) of the manual will be soon available for the LightCycler. For the moment I don't know what file...	grelierg	Sep 2, 2005 11:18 am
38	We hope to have all import formats ready by the end of septmeber. Untill then data can best be converted manually to the qBase format, and then be imported as...	hellemans_jan	Sep 2, 2005 11:21 am
39	I am generating data with a BioRad Icycler (ODM files). It appears I can only import excel files onto qBase. It is my understanding I need to convert the data...	jhunter016	Sep 6, 2005 2:19 pm
40	Apparently qBase problems encountered in Excel 2000 can be solved by installing Excel 2003! Dear Jan, I inform you that I loaded Excel 2003 instead of Excel...	hellemans_jan	Sep 20, 2005 2:28 pm
41	Is it possible for qBase to use the measured relative total RNA in each sample like a reference gene? Thanks, Cathy Richter...	Cathy cathyr1305	Oct 5, 2005 2:48 pm
42	Dear Jo and Jan, Is it possible to import data from ABI 7500 real time PCR machine? Where do I find the "qBase Analyser (Experiment Mode" program in the qBase...	ovearla	Oct 7, 2005 12:58 pm
43	Dear Ove, 1) ABI 7500 should be supported in qBase v1.1.0. A new import wizard will be released in version 1.2.0 that should cause less problems. 2) You don't...	hellemans_jan	Oct 7, 2005 1:13 pm
44	Dear Cathy, This is not possible. However, there is a workaround if you insist (see below). Please note that the use of total RNA for normalisation might...	Jo Vandesompele jvdesomp	Oct 7, 2005 2:00 pm
45	I just downloaded qBase and would like to use it for quantitation of qPCR reactions. I run duplex and triplex qPCRs on genomic DNA for genotyping purposes and...	Ivan J Delgado ivaandelgado	Oct 10, 2005 10:26 pm
46	Dear Ivan, In principle, qBase can handle any kind of relative quantification. You will have to import the different dyes as different runs though (pretending...	jvdesomp	Oct 11, 2005 5:05 am

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