"qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" by Hellemans et al. is...
Dear Jo I am begining a fairly large qPCR study, but I am concerned that we may not be able to be able to analyse or data to it's full potential if we get the...
... Of course! The sample maximization approach is only proposed to reduced inter-run variation when comparing samples. You are still able to compare genes...
Thank you for your reply Jo You mention the use of IRCs in your reply. Can you clarify some points regarding IRCs for me please. In the qBase tutorial, the...
... Indeed, because all samples fit on the same plate. ... Correct. ... There is no run-to-run variatio, because all samples are tested in the same plate....
Greetings, My last question was stupid. I have used Opticon Monitor to output Ct values for me, inserted these values into QBase format files, and then used...
Hello Im very new to the field of qRT-PCR analylis and would like to use qBase for my calculations. But i encountered a, lets call it "data organisation...
Can you also state the number of samples in each subgroup and number of PCR replicates? What are your inter-run calibrators? How many IRCs do you have? If...
Hello Jo Thank you for your quick response. I have 8 samples in each of the 4 subgoups. All the samples ran as duplicates. KO-plate setup: - triplicate...
Your experimental design seems perfect. I just wanted to note that you can also use the standards for the IRC procedure. If these are diluted cDNAs (pooled or...
QBase detects IRCs that are really not IRCs. They are normal samples. It only detects these false IRCs in the last Run I enter (I am entering 5 runs into my...
I have been able to solve the run time error "9" that occured while detecting IRCs after files had been imported. The solution was to make all gene and sample...
Hello Jo I think i found the problem. In my plate setup i used only the sample name as identifier (which of course was the same in the different runs for the...
Does the new version of qbase support Quantica (Techne) realtime machine? I note it is listed in the input data area but it won't allow me to import my excel...
qBase does not support Quantica export files yet. Please send us representative export files (and info on how you exported them) , and we will try to write an...
Dear Jo, I'm trying to validate a quiet large number of gene-expression datas with RT-PCR. I would like to compare the relative gene-expression of 4 groups: 3...
... If you don't use standards, you will have to assume the efficiency of your reactions, and you don't know the limit of quantification of your assay. If you...
A number of problems in qBase are caused because the program can not deal with gene names that can be interpreted as numbers or as dates. This problem can...
Is there any reason why my qBase won't save the Gene efficiency's I determine for my genes ... Not only this, but have to change the preferences to use gene...
I am new here and I have been trying to analyze a large set of data. I have my inter-run calibrator in my samples and I would like to analyze my samples so...
Hi, I've just installed qBase and am now trying to get it running. When I try to run the provided datasets and I click analyze, excel stops responding. I get a...
I have done a rt-pcr experiment with 3 house keeping genes and a gene of interest. The reference gene quality evaluation gives the following values: CV M...
The qBase paper (PMID: 17291332) contains a list with indicative values for acceptable CV and M values. Mean CV and M values lower than 25% and 0.5,...
I have done an rt-pcr experiment in a family with 2 patients and 2 control samples. 3 samples have normal values (final values after analysis in qbase) around...
Hi, Sorry to repost this from Nov 06, but I am hoping that in the interim someone has successfully tried qBase with the Mx4000 data format. ... Thank you, ...
Hi Friends Does anyone of you used RNAs(ribosomal, ncRNAs etc) as reference markers for normalisation of your expression data? If yes, which method did you...
Hi! I have been using the qBase v.1.2.2 to analyze my results. When I have tried to use more recent versions (i.e: v.1.3.5, and others), these give me a lot of...
Hi Joo What could be the reaon for M values higher than 1? I runned an analysis with 5 genes and all of them had values over 1. Another question: I have 17...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
