Dear Jo, I'm trying to validate a quiet large number of gene-expression datas with RT-PCR. I would like to compare the relative gene-expression of 4 groups: 3...
... If you don't use standards, you will have to assume the efficiency of your reactions, and you don't know the limit of quantification of your assay. If you...
A number of problems in qBase are caused because the program can not deal with gene names that can be interpreted as numbers or as dates. This problem can...
Is there any reason why my qBase won't save the Gene efficiency's I determine for my genes ... Not only this, but have to change the preferences to use gene...
I am new here and I have been trying to analyze a large set of data. I have my inter-run calibrator in my samples and I would like to analyze my samples so...
Hi, I've just installed qBase and am now trying to get it running. When I try to run the provided datasets and I click analyze, excel stops responding. I get a...
I have done a rt-pcr experiment with 3 house keeping genes and a gene of interest. The reference gene quality evaluation gives the following values: CV M...
The qBase paper (PMID: 17291332) contains a list with indicative values for acceptable CV and M values. Mean CV and M values lower than 25% and 0.5,...
I have done an rt-pcr experiment in a family with 2 patients and 2 control samples. 3 samples have normal values (final values after analysis in qbase) around...
Hi, Sorry to repost this from Nov 06, but I am hoping that in the interim someone has successfully tried qBase with the Mx4000 data format. ... Thank you, ...
Hi Friends Does anyone of you used RNAs(ribosomal, ncRNAs etc) as reference markers for normalisation of your expression data? If yes, which method did you...
Hi! I have been using the qBase v.1.2.2 to analyze my results. When I have tried to use more recent versions (i.e: v.1.3.5, and others), these give me a lot of...
Hi Joo What could be the reaon for M values higher than 1? I runned an analysis with 5 genes and all of them had values over 1. Another question: I have 17...
Dear Adrian, qBase's possibility to evaluate the stability of reference genes is intended to measure the stability of selected reference genes in your actual....
Dear Jan I have used geNorm for the initial tests and than qBASE because I believed is an advanced form of it! Is there a treshold for M values? My lowest is...
Compatibility of qBase with different versions of Excel Excel 2003: good (qBase is developed and tested in Excel 2003) Excel 2000 and XP: works on most PC's...
Hi I would be very grateful for some help with my experiments. I have been using SYBR Green to measure expression of my transgene (IL10) in adenoviral vector...
Dear Jo and Jan; I wrote one month ago asking you about some problems with the IRCs using qBase. We do not understand properly why I can not introduce my data...
Dear Jo and Jan, My name is Davina and I have a problem with the relative quantification of my experiment. It consist of 7 96-well plates with on each plate 1 ...
Dear colleagues, I am starting to analyze my real-time data with qBase. It looks great, but apparently I have a problem with IRCs. I do not have samples spread...
Anna, qBase can not deal with technical replicates spread across plates very well. It will consider this as an experiment too large to fit into one plate,...
Davina, Your setup with just one plate per gene does not resuire inter-run calibration. What is the purpose of your callibrators? qBase allows you to compare...
The qBase Browser can not deal with 10 or more experiments in a single project. This problem can be circumvented by putting the tenth (and following)...
The main difference between v1.2 and v1.3 of qBase is the switch from inter-run calibration based on Ct values to inter-run calibration based on normalized...
We run our qPCR studies on an ABI 7500. When using the ABI software for relative expression studies, the individual plates have to be imported into a relative...
When I want to start qBase , i f found some difficulties. After left clicking on the start qBase the MVB shows a message run-time error '1004' and said that...
You will have to unzip or extract the qBase.zip file. Apparently, you are working in the zip file after double clicking and opening it. Good luck. Jo...
Hi Have exactly the same runs repeated several times i.e. technical replicates in different runs though some are from different cDNA synthesis of the same RNA...
Hi I have ranked my genes with geNORM but when I analyse with qBASE the same set of data the M values and the evaluation of the reference genes is totally...
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