Dear Jo and Jan, My name is Davina and I have a problem with the relative quantification of my experiment. It consist of 7 96-well plates with on each plate 1 ...
Dear colleagues, I am starting to analyze my real-time data with qBase. It looks great, but apparently I have a problem with IRCs. I do not have samples spread...
Anna, qBase can not deal with technical replicates spread across plates very well. It will consider this as an experiment too large to fit into one plate,...
Davina, Your setup with just one plate per gene does not resuire inter-run calibration. What is the purpose of your callibrators? qBase allows you to compare...
The qBase Browser can not deal with 10 or more experiments in a single project. This problem can be circumvented by putting the tenth (and following)...
The main difference between v1.2 and v1.3 of qBase is the switch from inter-run calibration based on Ct values to inter-run calibration based on normalized...
We run our qPCR studies on an ABI 7500. When using the ABI software for relative expression studies, the individual plates have to be imported into a relative...
When I want to start qBase , i f found some difficulties. After left clicking on the start qBase the MVB shows a message run-time error '1004' and said that...
You will have to unzip or extract the qBase.zip file. Apparently, you are working in the zip file after double clicking and opening it. Good luck. Jo...
Hi Have exactly the same runs repeated several times i.e. technical replicates in different runs though some are from different cDNA synthesis of the same RNA...
Hi I have ranked my genes with geNORM but when I analyse with qBASE the same set of data the M values and the evaluation of the reference genes is totally...
Hi I have a problem to import run data to my experiment. Every times I try, a message : an error occurred because one of the column headers is not correct. My...
Hi I still have trouble to import my excel file for analysis. the same message (an error occurred because one of the column header is not correct. I tried to...
The importer for ABI relative quantification is designed for single runs. Therefore data from a study with multiple plates will probably result in errors. The...
Hi reading my first question again I realise that what I mean to say is that I have repeated identical runs in terms of samples (though some are from separate...
In principle inter-run calibration can be performed on Ct values or on normalized relative quantities. qBase performs IRC on NRQ's meaning that fresh cDNA...
G'day Jan, Thank you for the program. I have several problems. [1] I can import may data files into qBase but in the run editor view mode I can only see...
Hello I am a new user of qbase. When I try to initialize experiment one in the tutorial, qbase crashes. I am running excel 9.0 on windows xp. Any help is...
Hi. I tried to get familiar with the qbase software but I still have some problem. I have always a missing column (F2) for three run and also see 13 is always...
Hey, I am a new user of qBase. Have had good succes with doing the tutorial and find qBase a very usefull tool for my work, thank you very much for that. ...
Hi, I am currently designing a study with 3 genes (2 HKG, 1 gene of interest), and many samples and I want to use relative quantitation. The total number of...
Hi When adding experiment 10 to a project the experiment is added but also adds two runs in the run box as #N/A and get error message Run time error 13 type...
You don't need inter-run calibrators, because both samples of the pair are tested on the same plate, and you compare only ratios (pre/post) across plates. The...
qBase can not handle projects with more than 9 experiments properly (see item 250 in this forum). The workaround is to create an additional project and to put...
When analyzing NTC samples, normalization does not make sense. As a consequence normalized relative quantities for no template controls can end up everywhere,...
Having just downloaded the software I receive the following message - "run time error 438": object doesn't support this property or method - when clicking...
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