Hi, First let me say that qbase looks like one of the most user friendly programmes available for rtPCR data handling I have come across. Thank you for writing...
Dear, after reading the posted messages I could recreate all of the problems posted with importing data. I use a MyIQ-BioRad machine and my output files look...
Dear Dr. A. Schiel The manual/previously-messege-replies states that the import file should look EXACTLY like it does in the manual.. I've discovered that the...
Hello, I use a MyIQ software and I try to use qBase but with no success. The VHL example doesn't work: I can't analyse the VHL example. When I try to do it,...
Some users experience severe problems with qBase, and are not even able to analyze the VHL experiment example. This is a known bug of qBase which occurs only...
We just released a new version of qBase with new features and bug fixes, and an updated manual. We recommend to install the new version by going to the qBase...
Dear Jo and Jan, I want to compare qBASE to the Genex Macro from Bio-Rad. I have a problem to get qBase to analyse. We want to analyse the expression of 4...
Dear Marcel, You're on the right track! Your experimental design is perfect (each gene on another plate). If all goes well, qBase should provide you with ...
Hello, I tried to install the qbase software on my PC (Windows 98). I wasn't able to 'view certificate' and to install the digital certificate. I enabled all...
... The digital certificate is made to avoid working on an unsecure level (allow all macros) or to allow the qBase macros (medium security level) every time...
Hi, I would like to know if section 5 (Avaible imput formats) of the manual will be soon available for the LightCycler. For the moment I don't know what file...
We hope to have all import formats ready by the end of septmeber. Untill then data can best be converted manually to the qBase format, and then be imported as...
I am generating data with a BioRad Icycler (ODM files). It appears I can only import excel files onto qBase. It is my understanding I need to convert the data...
Apparently qBase problems encountered in Excel 2000 can be solved by installing Excel 2003! Dear Jan, I inform you that I loaded Excel 2003 instead of Excel...
Dear Jo and Jan, Is it possible to import data from ABI 7500 real time PCR machine? Where do I find the "qBase Analyser (Experiment Mode" program in the qBase...
Dear Ove, 1) ABI 7500 should be supported in qBase v1.1.0. A new import wizard will be released in version 1.2.0 that should cause less problems. 2) You don't...
Dear Cathy, This is not possible. However, there is a workaround if you insist (see below). Please note that the use of total RNA for normalisation might...
I just downloaded qBase and would like to use it for quantitation of qPCR reactions. I run duplex and triplex qPCRs on genomic DNA for genotyping purposes and...
Dear Ivan, In principle, qBase can handle any kind of relative quantification. You will have to import the different dyes as different runs though (pretending...
In their artical (Neuroscience letters (2003), 339, 62-66, Ramakers et. al.use their program LinRegPCR to define PCR efficienies based individual PCR...
Hi, Is it possible, in qBase, to make a relative quantification of mRNA´s in bacteria using the method mentioned in Fey et al: "Establishment of a Real-Time...
Hello. I have been using qBASE for some time and I was very happy with it. But the suddenly I started to get run time error messages. I can import my data and...
Hi i just download the last version of qBase and i am not able to make it work. when i try to open one of the demo data i got the following message: this...
Frederique Hilliou
hilliou@...
Nov 16, 2005 7:43 pm
51
Hello. I am using qBase to determine copy number of certain deletions in a chromosomal area. I have several housekeeping genes, genes within my deleted...
Dear Aaron, We also use qBase for gene copy number assessment. I suggest you to read Hoebeeck et al., Laboratory Investigations, 2005. STD is a sample type...
"run time error 1004" with "can not get the Average-properties for the WorksheetFunction-class" usually indicates that one of the cells for which qBase is...
I am new to qBase and am having major problems just getting started. I think I am having problems with the link that is suppose to be in the qBase browser. I...
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