Hi I have a problem to import run data to my experiment. Every times I try, a message : an error occurred because one of the column headers is not correct. My...
Hi I still have trouble to import my excel file for analysis. the same message (an error occurred because one of the column header is not correct. I tried to...
The importer for ABI relative quantification is designed for single runs. Therefore data from a study with multiple plates will probably result in errors. The...
Hi reading my first question again I realise that what I mean to say is that I have repeated identical runs in terms of samples (though some are from separate...
In principle inter-run calibration can be performed on Ct values or on normalized relative quantities. qBase performs IRC on NRQ's meaning that fresh cDNA...
G'day Jan, Thank you for the program. I have several problems. [1] I can import may data files into qBase but in the run editor view mode I can only see...
Hello I am a new user of qbase. When I try to initialize experiment one in the tutorial, qbase crashes. I am running excel 9.0 on windows xp. Any help is...
Hi. I tried to get familiar with the qbase software but I still have some problem. I have always a missing column (F2) for three run and also see 13 is always...
Hey, I am a new user of qBase. Have had good succes with doing the tutorial and find qBase a very usefull tool for my work, thank you very much for that. ...
Hi, I am currently designing a study with 3 genes (2 HKG, 1 gene of interest), and many samples and I want to use relative quantitation. The total number of...
Hi When adding experiment 10 to a project the experiment is added but also adds two runs in the run box as #N/A and get error message Run time error 13 type...
You don't need inter-run calibrators, because both samples of the pair are tested on the same plate, and you compare only ratios (pre/post) across plates. The...
qBase can not handle projects with more than 9 experiments properly (see item 250 in this forum). The workaround is to create an additional project and to put...
When analyzing NTC samples, normalization does not make sense. As a consequence normalized relative quantities for no template controls can end up everywhere,...
Having just downloaded the software I receive the following message - "run time error 438": object doesn't support this property or method - when clicking...
Dear Jo and Jan We have carried out an experiment in which we want to look at the expression of 3 GOIs and 2 HKGs in 5 tissue samples from 49 patients ie 3...
Hail, We set up a small experiment to become familiar with expanding experiments in qBase. Our first experiment analyzed 5 GOIs and 3 REFs for 6 samples...
Dear Claus, In qBase, and actually for any analysis of qPCR data, it is required to use the same set of reference genes for all samples. Selecting only the 2...
Dear Jo, I was using qBase successfully when it started to give errors in the raw data analysis (run-time 1004) (initialisation ok) , only with some excel...
When looking at the standard curves for the different genes the scale of the x axis is only from 1 to 10 lately and with such a small scale the data is not...
Hi, I am having trouble importing the results of a 384 well plate run on an ABI7900. According to the manual, i have made an excel file with the column...
The amplification plot in qBase is not generated by the qBase code, but merely an object in the Excell sheet. Therefore it can be manually changed just like...
Hi I cannot do quality control for inter run calibration because I don't have the part relative to run calibration in the "options" interface screen. Could...
I tried to load my expriment files from the stratagene Mx3000P, which qBASE should support. The loading of the files work fine, but I get "Runtime error 9 -...
Hello, A quick one: what RQ calculation method should I use if I have a single reference gene? I see that if I use the qBase method I get normalization factor...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
