Hi, In my study i have run 2 housekeeping genes and 2 genes of interest. Each gene was run on a 384 plate of cDNA samples. My problem is that several samples...
I have done a real-time-pcr experiment with 2 house keeping genes and two targetgenes of interest. I analysed the data with qBASE. The reference gene quality...
Dear Isabelle, The table form the paper was merely intended for guideline purposes, so I'm sure your reference genes are okay. Remember that the use of more ...
Hi all, what was the problem? I'm experiencing the same conflict of results... Does having excluded samples in qBase matter? Thanks Ferdinando ... dot be). ......
We are using samples from human blood which are stimulated with anti CD3CD28. When I tested several of these samples (using the similar startconcentrations), I...
Dear Tania Your genes look perfectly stable. The fact that the normalization factors are different between the 2 groups can be coincidental (not so many...
Hi there, I'm using LinReg for calculation of amplification efficiency by averaging across all samples for each gene. Is there a way to calculate the error...
I would use the mean efficiency with the standard error of the mean as the error. Jo ... I would use the mean efficiency with the standard error of the mean as...
Hi, I'm really stuck with the IRC selections in qBase, hope you can help me. I have a large number of samples and genes spread across 7 runs. Aside from the...
Hi I was wondering if it would be possible to do biological replicates for each gene instead of doing 2 or 3 replicates of each sample. i.e. give each...
Hi Jo, Hi Jans, I'm trying to analyze some data in qBase but I'm experimenting a problem (runtime error 1004) when I add more than 1 reference gene for ...
Dear Damon In principle, qBase will be able to do the calculations. While the normalized relative quantities will be correct, the error propagation will not be...
Hi, I have one more question about the inter run calibration performed by qbase. If I decied to only include IRC's for one or two control genes in my analysis...
Hi, I have one more question about the inter run calibration performed by qbase. If I decied to only include IRC's for one or two control genes in my analysis...
Hi, I have 2 questions: 1. I have samples with different quality I want to compare. I used 2 ref genes that are normally very stable in this tissue (we tested...
Hi Alessandra and all. I got this same problem: "Run-time error '1004': application-defined or object-defined error" in Excel 2000 SP3. Does upgrade to 2003...
Hi, I have started using QBase after the presentations I saw last week and the conference in Rhode Island. I use the 7900HT platform with either 384 well...
Dear Jo Would you please have a look to the questions bellow that rose during an internal meeting? 1) What is/are the advantage(s) to use the mean Ct for...
Answers inserted into the text. ... <aperesbota@...> wrote: Dear Jo Would you please have a look to the questions bellow that rose during an internal meeting? ...
I was wondering if there are any users that are converting there data to either log base 2 or log base 10 and how they are handling to conversions of the error...
hi everyone, i m not jst a new user of qbase, and also new in the lab. i did my quantification pcr on the bio-rad icycler and annlysed the data by the qBase....
Hello, I am concerned with difference among NFs for our experimental setup of two groups of tissue pools. The NFs are near 0.3 in one group, and 2.5 in the...
The NF and M are both related to the reference genes, but in a completely different way. The M value must be interpreted as a quality parameter for the...
Comments on question 1 Although you may have validated your reference genes before it is still advisible to verify the stability of these reference genes in ...
Hello, Firstly, thank you for the opportunity to use qBase. I have just started using it and I am having a problem when I get to the data analysis step. When I...
Hi, I have an experimental setup where I had to make three cDNA plates to fit all my samples in (duplicate), all have a 5-point dilution curve (triplicate) of...
The professional successor qBasePlus will have the option to apply target-run specific PCR efficiency correction (using a standard curve in each run/plate). ...
Hi there I have used geNORM to rank the genes I analyse and got good results. later I have analysed more genes on the same samples and I have introduced the...
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