Hi Alessandra and all. I got this same problem: "Run-time error '1004': application-defined or object-defined error" in Excel 2000 SP3. Does upgrade to 2003...
Hi, I have started using QBase after the presentations I saw last week and the conference in Rhode Island. I use the 7900HT platform with either 384 well...
Dear Jo Would you please have a look to the questions bellow that rose during an internal meeting? 1) What is/are the advantage(s) to use the mean Ct for...
Answers inserted into the text. ... <aperesbota@...> wrote: Dear Jo Would you please have a look to the questions bellow that rose during an internal meeting? ...
I was wondering if there are any users that are converting there data to either log base 2 or log base 10 and how they are handling to conversions of the error...
hi everyone, i m not jst a new user of qbase, and also new in the lab. i did my quantification pcr on the bio-rad icycler and annlysed the data by the qBase....
Hello, I am concerned with difference among NFs for our experimental setup of two groups of tissue pools. The NFs are near 0.3 in one group, and 2.5 in the...
The NF and M are both related to the reference genes, but in a completely different way. The M value must be interpreted as a quality parameter for the...
Comments on question 1 Although you may have validated your reference genes before it is still advisible to verify the stability of these reference genes in ...
Hello, Firstly, thank you for the opportunity to use qBase. I have just started using it and I am having a problem when I get to the data analysis step. When I...
Hi, I have an experimental setup where I had to make three cDNA plates to fit all my samples in (duplicate), all have a 5-point dilution curve (triplicate) of...
The professional successor qBasePlus will have the option to apply target-run specific PCR efficiency correction (using a standard curve in each run/plate). ...
Hi there I have used geNORM to rank the genes I analyse and got good results. later I have analysed more genes on the same samples and I have introduced the...
Hi, I have 2 questions for problems with qbase ! 1. Is qbase compatible with windows vista ? I have installed qbase on my laptop with windows vista but when I...
Dear all, We start a experiment and we planning to analyse it in qBASE. So, we starting reading the qBASE tutorial, 1 Initial experiment. But, we are with some...
Dear Marcelo 1. If you are going to use qBase(Plus) for data-analysis, there is no need to use the RQ setup, so AQ is perfect. 2a. In AQ setup, you don't...
I'm using qbase with Excel xp (2002) on a windows machine. I've initialized my data and performed raw data quality control. When I calculate relative...
qBase is not compatible with Excel 2007. We advise Microsoft Office 2003. Biogazelle will release qBasePlus in February 2008. This program is based on the...
I have 2 questions regarding elimination of samples: 1. We included 9 endogenous control genes in our qPCR setup (TaqMan low density arrays, 32 genes run i...
Hello, I am trying to find a way to compare the relative expression values of multiple genes. I gather from the qBase manual that it can be possible to view...
You could, but this will only provide you with an approximative comparison of genes, because your underlying assumption is that an equal copy number of any...
Hi, We did a analysis on qBASE and we found a problem in set up the proper biological replication. We have four biological replicates for each condition: ...
Hello Marcelo, qBase does not do any statistical analysis on biological replicates. The program treats samples with the same name as technical replicates. ...
Dear qBase user qBasePlus is the professional successor of the qBase software that accompanied the real-time PCR quantification framework described in ...
Hello, I am still using qbase (hoping to upgrade one of these days) I didn't think I could use the free version of biogazelle since I have 14 runs I wish to...
Dear Doris, We have stopped development of the old Excel based qBase in favor of the faster and more user friendly qBasePlus, and can therefore no longer...
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