Hello, I am still using qbase (hoping to upgrade one of these days) I didn't think I could use the free version of biogazelle since I have 14 runs I wish to...
Dear qBase user qBasePlus is the professional successor of the qBase software that accompanied the real-time PCR quantification framework described in ...
Hi, We did a analysis on qBASE and we found a problem in set up the proper biological replication. We have four biological replicates for each condition: ...
Hello, I am trying to find a way to compare the relative expression values of multiple genes. I gather from the qBase manual that it can be possible to view...
I have 2 questions regarding elimination of samples: 1. We included 9 endogenous control genes in our qPCR setup (TaqMan low density arrays, 32 genes run i...
I'm using qbase with Excel xp (2002) on a windows machine. I've initialized my data and performed raw data quality control. When I calculate relative...
Dear all, We start a experiment and we planning to analyse it in qBASE. So, we starting reading the qBASE tutorial, 1 Initial experiment. But, we are with some...
Hi, I have 2 questions for problems with qbase ! 1. Is qbase compatible with windows vista ? I have installed qbase on my laptop with windows vista but when I...
Hi, I have an experimental setup where I had to make three cDNA plates to fit all my samples in (duplicate), all have a 5-point dilution curve (triplicate) of...
Hello, Firstly, thank you for the opportunity to use qBase. I have just started using it and I am having a problem when I get to the data analysis step. When I...
Hi, I have 2 questions: 1. I have samples with different quality I want to compare. I used 2 ref genes that are normally very stable in this tissue (we tested...
Hello, I am concerned with difference among NFs for our experimental setup of two groups of tissue pools. The NFs are near 0.3 in one group, and 2.5 in the...
hi everyone, i m not jst a new user of qbase, and also new in the lab. i did my quantification pcr on the bio-rad icycler and annlysed the data by the qBase....
I was wondering if there are any users that are converting there data to either log base 2 or log base 10 and how they are handling to conversions of the error...
Hi, I have started using QBase after the presentations I saw last week and the conference in Rhode Island. I use the 7900HT platform with either 384 well...
Hi, I have one more question about the inter run calibration performed by qbase. If I decied to only include IRC's for one or two control genes in my analysis...
Hi I just downloaded qBase earlier this week, but I am having trouble getting the program started. The Excel program freezes everytime I try to analyze an...
Hi, I have been running real time reactions on ABI-7300. For one particular sample I had 2 replicates and ran both the replicates in duplicates. But both the...
Hi I was wondering if it would be possible to do biological replicates for each gene instead of doing 2 or 3 replicates of each sample. i.e. give each...
Hi Jo, Hi Jans, I'm trying to analyze some data in qBase but I'm experimenting a problem (runtime error 1004) when I add more than 1 reference gene for ...
Hi, I'm really stuck with the IRC selections in qBase, hope you can help me. I have a large number of samples and genes spread across 7 runs. Aside from the...
Hi there, I'm using LinReg for calculation of amplification efficiency by averaging across all samples for each gene. Is there a way to calculate the error...
We are using samples from human blood which are stimulated with anti CD3CD28. When I tested several of these samples (using the similar startconcentrations), I...
Hi I have ranked my genes with geNORM but when I analyse with qBASE the same set of data the M values and the evaluation of the reference genes is totally...
I have done a real-time-pcr experiment with 2 house keeping genes and two targetgenes of interest. I analysed the data with qBASE. The reference gene quality...
Hi, In my study i have run 2 housekeeping genes and 2 genes of interest. Each gene was run on a 384 plate of cDNA samples. My problem is that several samples...
I am using Cepheid and converting run files to qBase file format as described in manual. However, I have a housekeeping gene tested in each sample. Where...
I am running into a problem with the run editor view where it is either empty (shows the grid, but no gene/sample names) or shows only the upper left quadrant...
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