Dear Jo and Jan, Is it possible to import data from ABI 7500 real time PCR machine? Where do I find the "qBase Analyser (Experiment Mode" program in the qBase...
Dear Ove, 1) ABI 7500 should be supported in qBase v1.1.0. A new import wizard will be released in version 1.2.0 that should cause less problems. 2) You don't...
Dear Cathy, This is not possible. However, there is a workaround if you insist (see below). Please note that the use of total RNA for normalisation might...
I just downloaded qBase and would like to use it for quantitation of qPCR reactions. I run duplex and triplex qPCRs on genomic DNA for genotyping purposes and...
Dear Ivan, In principle, qBase can handle any kind of relative quantification. You will have to import the different dyes as different runs though (pretending...
In their artical (Neuroscience letters (2003), 339, 62-66, Ramakers et. al.use their program LinRegPCR to define PCR efficienies based individual PCR...
Hi, Is it possible, in qBase, to make a relative quantification of mRNA´s in bacteria using the method mentioned in Fey et al: "Establishment of a Real-Time...
Hello. I have been using qBASE for some time and I was very happy with it. But the suddenly I started to get run time error messages. I can import my data and...
Hi i just download the last version of qBase and i am not able to make it work. when i try to open one of the demo data i got the following message: this...
Frederique Hilliou
hilliou@...
Nov 16, 2005 7:43 pm
51
Hello. I am using qBase to determine copy number of certain deletions in a chromosomal area. I have several housekeeping genes, genes within my deleted...
Dear Aaron, We also use qBase for gene copy number assessment. I suggest you to read Hoebeeck et al., Laboratory Investigations, 2005. STD is a sample type...
"run time error 1004" with "can not get the Average-properties for the WorksheetFunction-class" usually indicates that one of the cells for which qBase is...
I am new to qBase and am having major problems just getting started. I think I am having problems with the link that is suppose to be in the qBase browser. I...
Problems like this result from the fact that the file information.xls is not open. Make sure the file is present in your qBase folder. Start qBase with...
We just released a new qBase version (1.2.3). This version is compatible with data export files from the iQ5 and LC480 machines. If you wish to install the new...
Hi, I want to import the rund data from my runs I made with a MX3005 P from Stratagene. qbase tells me that the format is not supported yet. I tried to import ...
Dear Martin, For the moment we have to few data to make a MX3005 P importer. It looks like with a little adjustment to your import file (see manual), it can...
... No. ... Indeed. (qbase should take of the calculations though...) ... No. ... Yes. We recommend at least 3 inter-run calibrators. Apart from unknown ...
I am a new user of Qbase. I have some problems: I would like to determine several calibrators in a same experiment (or in a same run too). In fact I would like...
Dear Sophie, qBase can only use one sample as a calibrator. However, it's very easy to modify the calibrator. Just go to the options in the menu, and change ...
Thank you very much for your answer. Best regards. Sophie LAFON Ingénieur R&D SCANELIS 23 chemin des Capelles 31076 Toulouse cedex 3 FRANCE Tel : +33 (0)5 34...
Hi I am looking for a software tool to analyse my data and calculate standard errors. I am using GAPDH and three target genes. I have 8 different treatments...
Yes, qBase can do this kind of calculations for you: select GAPDH as a reference gene and your negative control as the calibrator, rescale to the calibrator....
qBASE is renaming samples and standards as calibrators (IRC's) when I try to initialize the experiment. This is the smaller of two experiments that I have done...
I have an experiment that spans 8 plates (4 genes; Each gene is on 2 plates) with the appropriate gene specific standard (copy number) on each plate and...
qBase can only import Ct values, and not calculated quantities. The quantities that can be indicated during import are the starting quantities for the samples...
... As Jan indicated, qBase uses only Ct values and a gene specific efficiency to calculate relative quantities. Please note that qBase is a relative...
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