Hello. I am using qBase to determine copy number of certain deletions in a chromosomal area. I have several housekeeping genes, genes within my deleted...
Dear Aaron, We also use qBase for gene copy number assessment. I suggest you to read Hoebeeck et al., Laboratory Investigations, 2005. STD is a sample type...
"run time error 1004" with "can not get the Average-properties for the WorksheetFunction-class" usually indicates that one of the cells for which qBase is...
I am new to qBase and am having major problems just getting started. I think I am having problems with the link that is suppose to be in the qBase browser. I...
Problems like this result from the fact that the file information.xls is not open. Make sure the file is present in your qBase folder. Start qBase with...
We just released a new qBase version (1.2.3). This version is compatible with data export files from the iQ5 and LC480 machines. If you wish to install the new...
Hi, I want to import the rund data from my runs I made with a MX3005 P from Stratagene. qbase tells me that the format is not supported yet. I tried to import ...
Dear Martin, For the moment we have to few data to make a MX3005 P importer. It looks like with a little adjustment to your import file (see manual), it can...
... No. ... Indeed. (qbase should take of the calculations though...) ... No. ... Yes. We recommend at least 3 inter-run calibrators. Apart from unknown ...
I am a new user of Qbase. I have some problems: I would like to determine several calibrators in a same experiment (or in a same run too). In fact I would like...
Dear Sophie, qBase can only use one sample as a calibrator. However, it's very easy to modify the calibrator. Just go to the options in the menu, and change ...
Thank you very much for your answer. Best regards. Sophie LAFON Ingénieur R&D SCANELIS 23 chemin des Capelles 31076 Toulouse cedex 3 FRANCE Tel : +33 (0)5 34...
Hi I am looking for a software tool to analyse my data and calculate standard errors. I am using GAPDH and three target genes. I have 8 different treatments...
Yes, qBase can do this kind of calculations for you: select GAPDH as a reference gene and your negative control as the calibrator, rescale to the calibrator....
qBASE is renaming samples and standards as calibrators (IRC's) when I try to initialize the experiment. This is the smaller of two experiments that I have done...
I have an experiment that spans 8 plates (4 genes; Each gene is on 2 plates) with the appropriate gene specific standard (copy number) on each plate and...
qBase can only import Ct values, and not calculated quantities. The quantities that can be indicated during import are the starting quantities for the samples...
... As Jan indicated, qBase uses only Ct values and a gene specific efficiency to calculate relative quantities. Please note that qBase is a relative...
This is usually caused by one or more standards without a Ct value, or for which there is no quantity associated. Make sure there is a Ct and quantity for...
Hi there, as "Mike" reported on Feb.10, I have found the same issue in version 1.3.3 with IRC allocation. While trying to troubleshoot I noticed that with only...
Dear John, I have fixed an error in the allocation of IRCs. After having verified the corrected qBase version on a number of experiments that resulted in...
Great! Although I appreciate it being free, I hope you somehow plan to market qBase soon after the bugs get worked out, as there are few alternatives for...
... Nice effort on the scale of that experiment John!! I have struggled to deal with 4 genes over 8 96 well plates. This seems to be the only option being...
Hi all, the updated analyzer (Feb.15) which overwrites v.1.3.3 (Feb.10) was successful in properly allocating all calibrators in my experiment (50x ABI 7900...
Hi there, after successfully initializing and calculating relative quantities of my data I proceeded to the "Inter-run calibration" where during the process,...
Hi, I'm having problems for importing input data from our Corbett Rotor- Gene 3000. The procedure works for getting the No., Name, Type and Ct as specified on...
Hey Phil, I think qBase only calculates RELATIVE expression values (ie. derived directly from delta Ct's). Your standard curves by definition give you...
While John is right that qBase is a relative quantification program, the use of standards not necessarily implies that you are doing absolute quantification...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
