I been trying to get Qbase to work for me but have been having trouble. I am using an ABI machine (either the 7500 or the 7000). I export the results and edit...
The qBase importers are constructed to work on the export files directly. Therefore these files shouldn't be edited prior to import in qBase. Regards, Jan ... ...
Hi, I'm new to qBase and I'm having problems to start this program. I'm using Windows XP/Excel 2003. I've already checked that the Exel security level ist set...
Dear Stefan, This sounds like the typical problem in which qBase is launched from within a zip folder. Extract the qBase.zip file and launch qBase from the ...
Beste Bas, In versie 1.3.3 zitten inderdaad nog enkele bugs. Deze week zou er nog een update moeten uitkomen. Voor het aanpassen van staaltypes e.d. kan je...
Hi, I have just downloaded the qBase program and I was hoping to do a tutorial to get the hang of the way it works. The zip file looks like it includes data...
We just released a new version of qBase. Apart from a few new features, this version is mainly a major bug fix release. We recommend to install the new version...
If no data are shown in the results table, it can simply be caused because the focus in the sheet is too far to the bottom right. This can easily be solved by...
I am looking for software to compare biological replicate experiments. In the qBase manual it states that biological replicates cannot be analyzed using this...
Hi there qBase can do all the calculations. However, the statistical interpretation is not performed. For this, you need to export the qBase results and...
Hi Jo, Thanks for your responses both in this group and the QPCR group as well. I installed Qbase on my computer and when I try to work with the program by...
Hello all, I am absolutely new to qBASE. I have downloaded the new v1.3.4. Q1) can I import raw data in excel format from the opticon2 (MJ) and copy and paste...
... No. Please see qBase manual how to prepare input files. For some instruments you need to export the data into a txt, csv or xls file directly; for others,...
Hi The current qBase version does not perform any statistical test. However, you can easily export results, and then apply the proper statistics in your...
Hello Jan, Now, I use TDLA from Applied Biosystems for my research. My question is: Can I use qbase for TLDA? What instructions must I follow before use?...
Dear Eric Yes, qBase is compatible with low density arrays. The only weak point might be in the comparison of data from multiple plates. If you don't repeat a...
Dear Jo, Thanks for the help. Although I managed to open qBase and define the project, experiment and runs, I am having trouble being able to import my excel...
Hi there, First I want to give a short description of my experiment: I have 11 conditions and for each condition I have 6 samples so in total I have 66...
Hi there, I was playing with some data in qBase and when I tried to start the analysis an error popped up when the initialization started: The error was:...
Dear Janneke qBase uses only one gene specific efficiency. So you cannot use different efficiencies in different runs (plates). If the standard is exactly the...
... The standard in both runs is made of the same cDNA dilution standard. But I already saw that they are not the same for both plates...I already runned 2...
Hello, I am searching for a template to import excel sheet into Qbase; i have read the manual and i created four columns as following: #1 Number #2 Name (name...
Dear Charlotte Please find attached the expression stability values we calculated for the 10 human reference genes in 85 samples from 5 different tissue groups...
Hello, I am doing chromatin immunoprecipitation assay and I´m analyzing the immunoprecipitated fragments with quantitative PCR. I think I can use qBASE...
Dear Asantha You column headers to not exactly match the description in the manual. * Well OR Well / Set * Dye * Type OR Content * Label OR Description * C(T) ...
Hello. I'm new to qbase and I am having troubling determining the NF for my data. I am working with mice and using three reference genes, run three times in...
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