Hi there qBase can do all the calculations. However, the statistical interpretation is not performed. For this, you need to export the qBase results and...
Hi Jo, Thanks for your responses both in this group and the QPCR group as well. I installed Qbase on my computer and when I try to work with the program by...
Hello all, I am absolutely new to qBASE. I have downloaded the new v1.3.4. Q1) can I import raw data in excel format from the opticon2 (MJ) and copy and paste...
... No. Please see qBase manual how to prepare input files. For some instruments you need to export the data into a txt, csv or xls file directly; for others,...
Hi The current qBase version does not perform any statistical test. However, you can easily export results, and then apply the proper statistics in your...
Hello Jan, Now, I use TDLA from Applied Biosystems for my research. My question is: Can I use qbase for TLDA? What instructions must I follow before use?...
Dear Eric Yes, qBase is compatible with low density arrays. The only weak point might be in the comparison of data from multiple plates. If you don't repeat a...
Dear Jo, Thanks for the help. Although I managed to open qBase and define the project, experiment and runs, I am having trouble being able to import my excel...
Hi there, First I want to give a short description of my experiment: I have 11 conditions and for each condition I have 6 samples so in total I have 66...
Hi there, I was playing with some data in qBase and when I tried to start the analysis an error popped up when the initialization started: The error was:...
Dear Janneke qBase uses only one gene specific efficiency. So you cannot use different efficiencies in different runs (plates). If the standard is exactly the...
... The standard in both runs is made of the same cDNA dilution standard. But I already saw that they are not the same for both plates...I already runned 2...
Hello, I am searching for a template to import excel sheet into Qbase; i have read the manual and i created four columns as following: #1 Number #2 Name (name...
Dear Charlotte Please find attached the expression stability values we calculated for the 10 human reference genes in 85 samples from 5 different tissue groups...
Hello, I am doing chromatin immunoprecipitation assay and I´m analyzing the immunoprecipitated fragments with quantitative PCR. I think I can use qBASE...
Dear Asantha You column headers to not exactly match the description in the manual. * Well OR Well / Set * Dye * Type OR Content * Label OR Description * C(T) ...
Hello. I'm new to qbase and I am having troubling determining the NF for my data. I am working with mice and using three reference genes, run three times in...
Hi, could you please provide me with a full reference for the software for my poseter presentation to be presented at ATS 2006 (American Thoracic Society)....
Dear Ben, First of all, NFs (normalization factors) are not ratios, but geometric averages of the reference gene expression levels. Secondly, they should be...
Hi Jo, Thanx for your advice concerning the inter-run callibrators. I used 3 samples for that, but now I'am running out of cDNA for these 3 samples. I made new...
Dear Janneke I assume you want to measure more samples and compare these with previously measured samples. Just make new cDNA of the inter-run calibrators,...
Beste Jo, Na je antwoord op mijn vorige bericht heb ik nog 2 vragen. In het Nederlands kan ik mijn "probleem" duidelijker uitleggen. Omdat ik 3 samples als...
Hello everyone, and thanks again for all the help with my last question. I was wondering if any one could help me with some new questions I had about qbase. ...
Hi, I'm having problems with the calculation-part of the qBase tool. I have data for 4 genes (53 samples each) entered. There are three IRCs (inter run...
Dear Claudius, Please send the qBase export of your experiment (jvdesomp @ gmail . com), with indication in the mail of which reference genes you want to use....
Hi again, Sorry for asking a dumb question - but what do the results mean that I get for the selected reference genes? If I select 3 reference genes out of 4...
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