I'm new to this so I'm trying to take a few baby steps. So far I can't even stand up. The goal is to create and align many montages, but I can't even get a ...
Brian, Certainly the program shouldn't crash when creating a domain boundary or adjusting contrast on a domain, and I haven't seen it crash like that with my...
Richard, There was a problem with exporting large images as black in versions 1.0.5.2 and before. But this seems to be fixed in my v1.0.5.4. You'll have to...
Can't list objects more than once. This problem appears to be computer specific and only occurs on our newest computer with the new RAID disk drives. Loading...
Brian, The problem appears to be in the background thread that reads the section files and gathers the object list data. The thread is doing the same thing as...
Reconstruct Users, Frequently I'm asked whether Linux will run under LINUX and so I'm posting this email to the group to let everyone know that it apparently...
OK, ok. I need to start proofreading my emails... ... From: "John Fiala" <fiala@...> To: "Reconstruct Users" <reconstruct_users@yahoogroups.com> Sent:...
Dear All, I am a new user of the reconstruct software and I have a question: Is it possible to trace a single structure on a single section ? In my serial...
Ulysses, If you generate the object as a Sphere in 3D (or as Traces, Trace Midpoints, or Box) it will be visible. Go the 3D tab in the Series Options. John. ...
James, The approach I've used to make 3D animations of rotating scenes requires a little work: 1) Layout the scene you want and size the 3D Scene Window for...
Hey John, sorry i'm replying this late; i just checked my email. Thanks for making the cursors black and white, they work great. i just tested the new version ...
Dear all, is there any possibility to montage two and more domains within one section by a point to point routine, but not as described in chapter 10, where...
Tobias, Yes, the trick is to import the domain (temporarily) into the adjacent section, use the method in chapter 10, and then Record the transformation "from...
Dear John, As usual, I write you to suggest an improvement for reconstruct I think it will be useful if in the thumbnails it appeared the number of the section...
Hi, I am wanting to reconstruct confocal images which are of the format .pic. Is there any way of doing this using the Reconstruct software? Many thanks, Kara...
Kara, You'll need to convert your .pic format into a format Reconstruct understands: http://synapses.bu.edu/tools/formats.htm I don't know what software would...
Hi i am new to reconstruct. I am planning to use it for counting the unbiassed numbers of primordial forllicles in buffalo ovaries using 3- 5 micron sections...
Dr. Rao, Yes, you can. See the article at http://synapses.bu.edu/tools/sightings/jamia.pdf for some info on counting in 3D, and the user manual chapter 1...
Tobias, Sounds like you figured it out. For anyone else interested more specific information, I've added the protocol to chapter 10 of the users manual:...
Dear All, Thanks for the information on the file formatting for confocal files. I have successfully inserted my confocal images into Reconstruct, andI am at...
Kara, I was able to get a reasonable 3D picture using Traces if I significantly reduced the section thickness. But you just have too many small, irrelevant,...
Reconstruct Users, I have posted a new builld (v1.0.5.6) with minor changes on the users group: http://groups.yahoo.com/group/reconstruct_users/files/ This...
Aaron, I get what you mean, it would be nice to have a faster method for selecting a trace while wielding one of the drawing tools. Ctrl-left-mouse is...
Dear John, I would like to measure my 3D objects, which are spiny dendrites. I found Reconstruct has a function to measure volume of the whole 3D object. I...
Yoshiyuki, To measure a 3D distance, use the Z-trace tool to create the distance line and then display the distance in the Z-trace List. To make a measurement...
Reconstruct Users, I've posted a new version (1.0.5.7) of Reconstruct at http://groups.yahoo.com/group/reconstruct_users/files/ This version fixes (for real...
Dear Dr Fiala, Thank you for your helpful email yesterday - I have managed to find my way to the user group. I have encountered a few difficulties which are...
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