Dear All, Thanks for the information on the file formatting for confocal files. I have successfully inserted my confocal images into Reconstruct, andI am at...
Kara, I was able to get a reasonable 3D picture using Traces if I significantly reduced the section thickness. But you just have too many small, irrelevant,...
Reconstruct Users, I have posted a new builld (v1.0.5.6) with minor changes on the users group: http://groups.yahoo.com/group/reconstruct_users/files/ This...
Aaron, I get what you mean, it would be nice to have a faster method for selecting a trace while wielding one of the drawing tools. Ctrl-left-mouse is...
Dear John, I would like to measure my 3D objects, which are spiny dendrites. I found Reconstruct has a function to measure volume of the whole 3D object. I...
Yoshiyuki, To measure a 3D distance, use the Z-trace tool to create the distance line and then display the distance in the Z-trace List. To make a measurement...
Reconstruct Users, I've posted a new version (1.0.5.7) of Reconstruct at http://groups.yahoo.com/group/reconstruct_users/files/ This version fixes (for real...
Dear Dr Fiala, Thank you for your helpful email yesterday - I have managed to find my way to the user group. I have encountered a few difficulties which are...
Jenny, If you downloaded 1.0.5.0 from synapses.bu.edu, you can find the example series in the Reconstruct installation directory, normally C:\Program...
Dear John Thanks for your help yesterday - I successfully used your example but was still unable to reconstruct using my own sections..until I changed ...
Is there a way to reconstruct single vesicles within an active zone? I have tried this with the IGL Trace but was unsuccessful, does Reconstruct have any new...
you don't have to use virtual pc just for reconstruct. if you have osX operating system in your mac, i'm sure you can run reconstruct with wine. that way your...
Hi pirol1983, Have you tried this yourself? As I understand it, on the Mac, Wine runs on top of x86 emulation, which means an app running on Wine on a Mac...
Hi davi, I am actually running reconstruct with wine but on a linux PC. you are right, MACs use a totally different architechture, but i thought since their...
Jianli, Here's my reconstruction (den1.jpg, attached) -- it appears to be a fair reproduction of the stack image you send me (cell72n.jpg, also attached). To...
I have a problem with calibration and measurement of a 3D model. I have a sequence of 10 em's of 0.1 micron thickness. The sequence has been calibrated as...
I am making progress with my use of Reconstruct - there's still a lot to learn, however, two problems keep cropping up: 1) tracing branching structures seems...
Jenny, In order for the surfacing to be correct, traces of an object within a section must not intersect or overlap, or be duplicated. For details see:...
A new version of Reconstruct (1.0.6.0) is available from the Users Group (http://groups.yahoo.com/group/reconstruct_users/files/) or from synapses.bu.edu...
Hi John, We're putting together a workstation to (among other things) display Reconstruct series on a 30" monitor at 2650x1600 resolution. We'd like to be...
Dear All, I am reconstructing 3D images of filled pyramidal cells with the main objective of analysing dendrites/ dendritic spines. As I wish to compare...
Hello all reconstructers!! I was wondering if anybody had used reconstruct on Macs OS X. I use it on a PC but would love for it to run on my Mac Powerbook... ...
Reconstruct Users, A new version (1.0.6.1) is posted on the users group: http://groups.yahoo.com/group/reconstruct_users/ The two main fixes are: 1) Automatic...
Hi! I'm using Reconstruct since one and a half month and very satisfied with it. It's stable, fast, offers good possibilities and the 3D rendering is very...
Paul, Academic licenses for 3DS Max 7 for less than $700 appear to still be available from www.creationengine.com The "relax" modifier in 3DS Max has worked...
John, Paul, other Reconstructers, Just want to add to this thread about getting discounted versions of 3DS Max, current version 8 by Autodesk. In addition to...
Dear Richard, I'm very happily surprised by several useful reactions from around the world coming in very swiftly after i posted my question. I asked all...
I am a new user of reconstruct. Recently I took the pictures of the paraffin sections, but after I did the 3D reconstruction, I found that the projects I made...
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