You'll have to explain what you mean by "save traced point coordinates". ... From: crepatas To: reconstruct_users@yahoogroups.com Sent: Thursday, April 13,...
Crepatas, Correct. But be aware that the values in the section files are subject to the transformations encoded there. In other words, to get the actual...
Has anyone had this problem? What does the error message mean? It has recently started happening when I try to align sections using about 50 selected traces....
Jenny, Your alignment should be possible even with 50 traces. If the error is repeatable (i.e. it always happens on the same section with the same traces...
Jenny, I reproduced this error when I selected all the traces for alignment. I'll try to fix it soon. It may be specific to the use of Rigid. You might try...
Reconstruct Users, I've posted a new version that fixes the atan2 DOMAIN error crash that Jenny encountered when using Align Rigid. Even if you don't have that...
Reconstruct Users, I've posted a new version at: http://groups.yahoo.com/group/reconstruct_users/files This version includes 3 new features: 1) An orthographic...
We are testing Reconstruct to see if we can use it with whole body images to create 3D images. Yesterday I had good success with aligning 8 images. Each image...
Mark, No, it is not possible to execute an alignment in Reconstruct without user input, but in your case it may be possible to automate some aspects of the...
Reconstruct Users, A new version is available at: http://groups.yahoo.com/group/reconstruct_users/files/ This version adds some smoothing options not available...
Reconstruct Users, A new version is available at: http://groups.yahoo.com/group/reconstruct_users/files with two enhancements since version v1.0.6.8: 1) Fixed...
Reconstruct Users, I have posted a new build at: http://groups.yahoo.com/group/reconstruct_users/files/ This version incorporates an enhancement by Ju Lu that...
Hello, I was curious if there was a straightforward way to alter a group of images in photoshop simultaneously (or, alter one image, and set all the images in...
Hi Tyler, That option exists in Photoshop. I am not an expert, so I will describe the way I do, but probably there are faster or better ways to do it: - Create...
Reconstruct Users, A new version posted on the users group at: http://groups.yahoo.com/group/reconstruct_users/files makes the entire trace palette editable....
Reconstruct Users, I have posted a new version (1.0.7.4) of Reconstruct at: http://groups.yahoo.com/group/reconstruct_users/files that includes some...
You are going to need to calibrate your sections. Read the calibration chapter in the manual for more info. If you don't have a scale bar or grid to...
I don't use Photoshop much but I use other similar programs. What you want to do is often called "batch processing." A Google search for "Photoshop" and...
Hi, I was recently introduced to this really great program, and was attempting to use it to do some traces from muticolor (3 channels) confocal stacks using...
Hello, I'm a new user with this great program, and recently ran into a problem. In dealing with the thickness of my TEM sections, I noticed some parts are...
Jean, Thanks for pointing out the limitation on the Hue parameter. I'll look into implementing a solution. Originally, I used RGB parameters but it was very...
Shannon, No. When using serial sections you should rely on the fact that any thinning in one part of one section will be offset by a corresponding (after...
Also Shannon, how are you estimating section thickness? Are you using the cylindrical diameters method? If not, you may want to read the following paper - pdf...
Hi John, (Sorry if you receive this message a second time) Thanks a lot for your quick answer and the very helpful piece of advice concerning shortcut usage. I...
Jean, Try version 1.0.7.7 just posted at: http://groups.yahoo.com/group/reconstruct_users/files/ I think this fixes the hue wrap around issue. Also it include...
Hi John, Awesome! Thanks for taking care of the hue issue so fast! It works perfectly now. I will now ask Ju how to stop the wildfire before it reaches the end...
Hi John This a very basic problem and probably down to my ineptitude but I am having problems when exporting a single montaged section. I have tried exporting...
Steve, The image is not zipped. Try opening the new series created by the export operation. You can then see the image and locate the file name from the domain...
A description of the project: I'm trying to reconstruct certain parts of the brain (subthalamic nucleus, red nucleus, etc.) by cutting human brain blocks and...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
