We are testing Reconstruct to see if we can use it with whole body images to create 3D images. Yesterday I had good success with aligning 8 images. Each image...
Mark, No, it is not possible to execute an alignment in Reconstruct without user input, but in your case it may be possible to automate some aspects of the...
Reconstruct Users, A new version is available at: http://groups.yahoo.com/group/reconstruct_users/files/ This version adds some smoothing options not available...
Reconstruct Users, A new version is available at: http://groups.yahoo.com/group/reconstruct_users/files with two enhancements since version v1.0.6.8: 1) Fixed...
Reconstruct Users, I have posted a new build at: http://groups.yahoo.com/group/reconstruct_users/files/ This version incorporates an enhancement by Ju Lu that...
Hello, I was curious if there was a straightforward way to alter a group of images in photoshop simultaneously (or, alter one image, and set all the images in...
Hi Tyler, That option exists in Photoshop. I am not an expert, so I will describe the way I do, but probably there are faster or better ways to do it: - Create...
Reconstruct Users, A new version posted on the users group at: http://groups.yahoo.com/group/reconstruct_users/files makes the entire trace palette editable....
Reconstruct Users, I have posted a new version (1.0.7.4) of Reconstruct at: http://groups.yahoo.com/group/reconstruct_users/files that includes some...
You are going to need to calibrate your sections. Read the calibration chapter in the manual for more info. If you don't have a scale bar or grid to...
I don't use Photoshop much but I use other similar programs. What you want to do is often called "batch processing." A Google search for "Photoshop" and...
Hi, I was recently introduced to this really great program, and was attempting to use it to do some traces from muticolor (3 channels) confocal stacks using...
Hello, I'm a new user with this great program, and recently ran into a problem. In dealing with the thickness of my TEM sections, I noticed some parts are...
Jean, Thanks for pointing out the limitation on the Hue parameter. I'll look into implementing a solution. Originally, I used RGB parameters but it was very...
Shannon, No. When using serial sections you should rely on the fact that any thinning in one part of one section will be offset by a corresponding (after...
Also Shannon, how are you estimating section thickness? Are you using the cylindrical diameters method? If not, you may want to read the following paper - pdf...
Hi John, (Sorry if you receive this message a second time) Thanks a lot for your quick answer and the very helpful piece of advice concerning shortcut usage. I...
Jean, Try version 1.0.7.7 just posted at: http://groups.yahoo.com/group/reconstruct_users/files/ I think this fixes the hue wrap around issue. Also it include...
Hi John, Awesome! Thanks for taking care of the hue issue so fast! It works perfectly now. I will now ask Ju how to stop the wildfire before it reaches the end...
Hi John This a very basic problem and probably down to my ineptitude but I am having problems when exporting a single montaged section. I have tried exporting...
Steve, The image is not zipped. Try opening the new series created by the export operation. You can then see the image and locate the file name from the domain...
A description of the project: I'm trying to reconstruct certain parts of the brain (subthalamic nucleus, red nucleus, etc.) by cutting human brain blocks and...
I think I know how to fix this now after reading the FAQ. How do I change the area of a trace, namely from negative to positive? ... taking ... section...
Hello, I have been working with dendritic spines 3D reconstructions and I would like to experiment with "morphological averaging". Considering each spine as a...
Dear John, What happened to synapse website, http://www.synapses.mcg.edu/ ? I can not access it for a while. It is one of my favorites. Jianli fiala_jc...
Dear John, I'm wondering how one could extract from the Tranfrom element in the section data files the exact tranformation of the contours and/or images. The...
Elaine, The transformation equations are given in the Fiala & Harris, 2001, paper: http://synapses.bu.edu/tools/sightings/jamia.pdf Let me know if you need...
Apologies for not searching the archives first.... I am on the point of leaving for a weeks' holiday, but my boss wants me to ask this question before I go...
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