Dear John, I'm wondering how one could extract from the Tranfrom element in the section data files the exact tranformation of the contours and/or images. The...
Elaine, The transformation equations are given in the Fiala & Harris, 2001, paper: http://synapses.bu.edu/tools/sightings/jamia.pdf Let me know if you need...
Apologies for not searching the archives first.... I am on the point of leaving for a weeks' holiday, but my boss wants me to ask this question before I go...
Cornelia, It depends on your traces, especially how many trace segments you create. You need enough segments so that some segments are close to one branch...
I am doing point alignment of 800 images from light microscopy as I have no fiducial markers in my specimen. The first 50 images can be very well aligned, but...
mfriis2204, Try to follow the alignment tips given in: http://synapses.bu.edu/tools/chapter10.pdf Since you can detect the rotational offset it is also...
Robert, Surface area is the length of each trace times the section thickness summed over all traces of the object, while flat area treats open and closed...
Hi, John. When I click _Object_ and then _List objects_, reconstruct just stays at 'Reading.' When I quit Reconstruct, and then re-install it from the Yahoo...
It's probably not you, it's probably your computer. I've noticed that every once in a while this happens on my computer, unexpectedly the Object List reading...
Dear All Hi , I am looking for a suitable pen mouse to use that in the reconstruct software, i would like to sketch and drowing tracees directly on screen, I...
Hi Reza, I've been using Reconstruct for several years now with a Wacom Cintiq digitizing pad. The Cintiq has an embedded flat panel screen so you can draw...
Hello all quick question, I have just aligned a series of EM images (65 images in stack, ~ 100Mb, each). I am trying to export the output files from ...
BobbyK, You can search the Messages on the Users Group for an answer to this question: http://tech.groups.yahoo.com/group/reconstruct_users/message/58 Or you...
Is there a way to make the 3D images (e.g. a reconstructed dendrite) look 'smoother' without having to output to a different program? Reconstruct and Photoshop...
Yes. The biggest issue for smoothing is how good the alignment is. But when there are a few misalignments these can sometimes be eliminated by adjusting the...
Brian, If you mean the centroids of the traces for an object: Turn on the centroids and z-position output in the Trace List from the Series Options, and then...
I also have this problem with my computer as well. I tried to quit all the programs but it still didn't work. Normally I restart Windows and reopen the ...
Hi John First of all, thank you for making this great software available for the people involve in 3D reconstructions. Well John, I have a few problems with...
Hi John First of all, thank you for making this great software available for the people involve in 3D reconstructions. Well John, I have a few problems with...
Demian, The wildfire region growing is based on the pixel intensities displayed on the screen and can make very complex traces with many segments that carry...
Dear John Thank you for your comments, I'll take a look at that. The merge works fine, the only problem is when using wildfire several times then the program...
Thank you very much for such a soft...but... It could be very interesting to be able to import images files from photoshop ( tif, jpeg, and why not PSD)where...
Reconstruct Users, A new version of the software is available at: http://synapses.bu.edu/tools/download.htm This version includes built-in HTML help files so...
Reconstruct Users, I have posted an update that includes the following new features: 1) Wildfires: The appearance of the Autotracing tab has changed. All...
Is there a transformation between the coordinates reconstruct uses and the coordinates used in the output image. This is a problem because as you remember...
Reconstruct does not flip the export image, if that's what you mean. When you export the image and open the series created by the export the images are all...
Dear John, For some reason, the Ctrol+m shortcut for the merge command does not work in my computer. It does in other computers, so it is not a problem of the...
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