Dear All Hi , I am looking for a suitable pen mouse to use that in the reconstruct software, i would like to sketch and drowing tracees directly on screen, I...
Hi Reza, I've been using Reconstruct for several years now with a Wacom Cintiq digitizing pad. The Cintiq has an embedded flat panel screen so you can draw...
Hello all quick question, I have just aligned a series of EM images (65 images in stack, ~ 100Mb, each). I am trying to export the output files from ...
BobbyK, You can search the Messages on the Users Group for an answer to this question: http://tech.groups.yahoo.com/group/reconstruct_users/message/58 Or you...
Is there a way to make the 3D images (e.g. a reconstructed dendrite) look 'smoother' without having to output to a different program? Reconstruct and Photoshop...
Yes. The biggest issue for smoothing is how good the alignment is. But when there are a few misalignments these can sometimes be eliminated by adjusting the...
Brian, If you mean the centroids of the traces for an object: Turn on the centroids and z-position output in the Trace List from the Series Options, and then...
I also have this problem with my computer as well. I tried to quit all the programs but it still didn't work. Normally I restart Windows and reopen the ...
Hi John First of all, thank you for making this great software available for the people involve in 3D reconstructions. Well John, I have a few problems with...
Hi John First of all, thank you for making this great software available for the people involve in 3D reconstructions. Well John, I have a few problems with...
Demian, The wildfire region growing is based on the pixel intensities displayed on the screen and can make very complex traces with many segments that carry...
Dear John Thank you for your comments, I'll take a look at that. The merge works fine, the only problem is when using wildfire several times then the program...
Thank you very much for such a soft...but... It could be very interesting to be able to import images files from photoshop ( tif, jpeg, and why not PSD)where...
Reconstruct Users, A new version of the software is available at: http://synapses.bu.edu/tools/download.htm This version includes built-in HTML help files so...
Reconstruct Users, I have posted an update that includes the following new features: 1) Wildfires: The appearance of the Autotracing tab has changed. All...
Is there a transformation between the coordinates reconstruct uses and the coordinates used in the output image. This is a problem because as you remember...
Reconstruct does not flip the export image, if that's what you mean. When you export the image and open the series created by the export the images are all...
Dear John, For some reason, the Ctrol+m shortcut for the merge command does not work in my computer. It does in other computers, so it is not a problem of the...
Jon, Perhaps there's a hotkey definition for ctrl+M in Windows. You'll need to either track this down and undefine it, or just resort to using Alt-t-m instead....
Hi John, When the images are exported they are shown with the identity transformation, but there is also a black border around the image which according to the...
Quasi426, The position of the image relative to the black border would be determined by the original transformation used to align the images. Note that this...
John, I did not understand the Alt-t-m command, but you gave me the idea of trying Ctrl+Alt+m and that works! Yes, it is another key, but it is fine, at least...
Reconstruct Users, The latest file uploaded to the users group fixes a few bugs related to accessing image files in the series. These were mostly annoyances in...
Hi, I am a new Reconstruct user. I mistakenly saved my .TIFF files as 16- bit rather than 8-bit images. Now when I try to load images into a series,...
Hi shhhhhhhhhhquiet, If you have Photoshop, just make a new action (New action from Action tab in History/Actions window), then Record: 1) open the 16-bit...
Hi, Another good application to do that is ImageJ (it's free too). What you need to do is just to convert one image whilst recording a macro. Then tune the...
Thanks - I just realized that LSM510 will let you open converted TIFF's and will batch-convert for you. Kind of makes me feel pretty dumb for cut-and-pasting...
Hi, I am trying to figure out the reference point for the max X and max Y measurements in the Trace lists dialogue. Is this X and Y with respect to the...
Alexis, The X and Y coordinates of traces and objects are defined relative to an arbitrary origin around which you positioned your data. In other words, there...
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