Hi, I am a new Reconstruct user. I mistakenly saved my .TIFF files as 16- bit rather than 8-bit images. Now when I try to load images into a series,...
Hi shhhhhhhhhhquiet, If you have Photoshop, just make a new action (New action from Action tab in History/Actions window), then Record: 1) open the 16-bit...
Hi, Another good application to do that is ImageJ (it's free too). What you need to do is just to convert one image whilst recording a macro. Then tune the...
Thanks - I just realized that LSM510 will let you open converted TIFF's and will batch-convert for you. Kind of makes me feel pretty dumb for cut-and-pasting...
Hi, I am trying to figure out the reference point for the max X and max Y measurements in the Trace lists dialogue. Is this X and Y with respect to the...
Alexis, The X and Y coordinates of traces and objects are defined relative to an arbitrary origin around which you positioned your data. In other words, there...
Hi, John, I do not know if it is hard to implement this function in your next version of reconstruct software. I think it is very useful to use mouse to move...
Jianli Li, Have you tried using Correlation (Ctrl \) at low magnification? This should give a quick translational alignment. Given the way Reconstruct...
Hi, I keep getting this message, usually after clicking on "list objects" or during adding objects to scene. I thought my hard drive might be getting too full,...
Jenny, If you're using version 1.0.8.0 - 1.0.8.4, I suggest you try upgrading to version 1.0.8.6 (on the Users Group). Those other versions seem to have...
Hi John, Thanks, as ever, for your speedy response. I must admit I checked for an update a couple of days ago, but hadn't seen the 1.0.8.6 yet. I have...
Hi John, Happy New Year! And thank you for making such a useful program freely available. Would it be possible to tell Reconstruct to generate individual...
To calculate the average thickness of a 2D trace, would it be possible to do the following: Subtract Min X from Max X, giving you the average horizontal...
Alexis, From a longitundinal section through the center of a dendrite, like the image you sent me, the mean width of the segment is the area divided by the...
I would like to verify that the distance measurement is from the center point of the 1st object to the center point of the 2nd object. Thanks, Angelica...
Angelica, I'm not sure what you mean by "distance tool", but if you're asking about the measurements in the Distances List those are distances between the...
Have recently returned to some older data which I produced in October 2005 on my present computer, and am having difficulty in running it. because of the...
Robert, That message appears to be coming from your graphics accelerator hardware. Apparently the OpenGL commands are not able to be executed by the hardware...
Hi, John, I remember in the old version of reconstruct. There is a tool to merger VRML files. But I could not find it in the new version. Sorry for posting...
Jianli, You can still use the VRML merge program located at: http://synapse-web.org/tools/index.stm but this only works on VRML 1.0 files, so you'll need to...
Hello, I have the same exact question as the post that I've copied below, which I can't find an answer to. I'm specifically wondering if the Reconstruct...
Caroline, Reconstruct does not automatically detect the pixel size (e.g. in micrometers) of your image. If you know the pixel size in micrometers just enter...
Hello again John, Thank you so much for your help--now I'm confident in my xy distance measures. However, I have one more basic question about calibration, on...
Caroline, Use the same units for section thickness as for pixel size, in this case, 1 micron. The program should tell you what units are expected when you...
Dear Dr. Fiala, Thank you very much for releasing this great program that saved me a lot of time and frustration. I am now trying to extract the medial axis ...
Thomas, There is a bug in the Boissonant surfacing algorithm that sometimes leaves interior faces. Unfortunately, no resources have been directed at fixing...
Hi John, I'm using the z-trace tool to trace segments of dendrites, and the length measurement listed in the table is the most important piece of data coming...
Caroline, Perhaps you were using the Point-by-Point tracing tool rather than the Z-tool, by mistake? The Point-by-point tool will always flatten the trace to...
Hi John, Actually, I am using the z-trace tool. And the z-traces appear 3-dimensional in the correct way when I first add them to the scene, but when I close...
Caroline, Thanks for this additional information. I see now that there is a bug in how the program writes out the section numbers for z-traces that caused the...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
