Hi John, I'm using the z-trace tool to trace segments of dendrites, and the length measurement listed in the table is the most important piece of data coming...
Caroline, Perhaps you were using the Point-by-Point tracing tool rather than the Z-tool, by mistake? The Point-by-point tool will always flatten the trace to...
Hi John, Actually, I am using the z-trace tool. And the z-traces appear 3-dimensional in the correct way when I first add them to the scene, but when I close...
Caroline, Thanks for this additional information. I see now that there is a bug in how the program writes out the section numbers for z-traces that caused the...
Reconstruct Users, I've posted a new version in the files section of the users group: http://tech.groups.yahoo.com/group/reconstruct_users/files/ that fixes...
Dear all, is there anyone know how to presentations Boissonat surface drawn with front and back faces in wireframe mode? I saw one example in the manual, but...
Bin, Uncheck the Fill boxes in the Color/Transparency dialog (Object List Scene menu) after adding your object to the 3D Scene. John. ... From: binma2 To:...
hi John, Thank you for the message.Now I continue on the Z trace. 1.since I have not regular objects, I use Z-Trace at midpoint to geneate Z trace to smooth...
Bin, I recommend you find a z-trace/filter length combination that gives the best smoothing in Reconstruct, then generate 3D using face normals and export VRML...
Jason, Processor speed and amount of memory are not critical to the performance of Reconstruct, but the 7200 RPM SATA drives will help. Even better would be...
I am having difficulties setting parameters for wild fire. Having prepared some outlines in Photoshop, am having difficulty in getting Reconstruct to recognise...
Robert, Setting wildfire stop criteria to "hue differs by 10" is sufficient to distinguish yellow from brown, but if you're tracing against another background...
The difficulty I am facing is the alignment of one of my images to the next one. After putting the points on both images, I went to Align and clicked Linear....
HI there! I have the latest version of Reconstruct and it was working great but when I tried opening a file I had already made my traces on and had a great 3D...
Carolina, I don't really understand the problem you're having, but it sounds like it might be only in the 3D scene window, in which case it may be a problem...
Hi, John, Thanks for your help on how to merge multiple VRML files. Now there is another minor question. As reconstructed dendritic branch gets longer and...
Jianli, Use the z-trace to measure along the center line of the dendrite. See also the FAQ entry about measurements: http://synapses.bu.edu/tools/FAQ.htm#300 ...
Dear reconstrct users, I have a question not relate to software directly. Recently I am studing the divergence of single axon button. All reference I can find...
The work I think is on par with the references below is Sugihara and Shinoda's group has looked at both olivary and pontine afferents to the cerebellum. It is...
Per Andersen had that PNAS paper in 2003, looking at varicosity spacing in cerebellum and hippocampus. Also there was a Deng and Dunaevsky paper not too long...
Thanks for responses especially Alexis and Dan. Suguhrara,shinoda and Per Andersen's work is terrific but I think they are focusing on divergent output of...
Reconstruct Users, A new build v1.0.8.8 is available at: http://tech.groups.yahoo.com/group/reconstruct_users/files This build adds the ability to turn off...
Hi, John, I saw the option in domain|attributes but I did not see the effects on display after I uncheck green and blue. Could you write a sentence how to use...
Jainli, Strange, the effect is immediate (after clicking the OK button) for me. I also tried it on your cell72n series data (cell72n.034.tif) and unchecking...
Hi, John, Thank you very much and the problem solved. I did not realize I have to click red/green/blue twice to uncheck the color setting. This is a very cool...
I have a couple of questions: 1 – I am reconstructing data from various confocal image stacks which I have to piece together like a huge 3-D jigsaw puzzle. I...
Debbie, The acos DOMAIN error arises when the Boissonnat surfacing algorithm encounters invalid trace data, most likely traces that overlap within the section....
Hi, I have been using Reconstruct for some time now - but for the quantification of dendritic spine morphology. Briefly, I import confocal stack maximum...
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