The difficulty I am facing is the alignment of one of my images to the next one. After putting the points on both images, I went to Align and clicked Linear....
HI there! I have the latest version of Reconstruct and it was working great but when I tried opening a file I had already made my traces on and had a great 3D...
Carolina, I don't really understand the problem you're having, but it sounds like it might be only in the 3D scene window, in which case it may be a problem...
Hi, John, Thanks for your help on how to merge multiple VRML files. Now there is another minor question. As reconstructed dendritic branch gets longer and...
Jianli, Use the z-trace to measure along the center line of the dendrite. See also the FAQ entry about measurements: http://synapses.bu.edu/tools/FAQ.htm#300 ...
Dear reconstrct users, I have a question not relate to software directly. Recently I am studing the divergence of single axon button. All reference I can find...
The work I think is on par with the references below is Sugihara and Shinoda's group has looked at both olivary and pontine afferents to the cerebellum. It is...
Per Andersen had that PNAS paper in 2003, looking at varicosity spacing in cerebellum and hippocampus. Also there was a Deng and Dunaevsky paper not too long...
Thanks for responses especially Alexis and Dan. Suguhrara,shinoda and Per Andersen's work is terrific but I think they are focusing on divergent output of...
Reconstruct Users, A new build v1.0.8.8 is available at: http://tech.groups.yahoo.com/group/reconstruct_users/files This build adds the ability to turn off...
Hi, John, I saw the option in domain|attributes but I did not see the effects on display after I uncheck green and blue. Could you write a sentence how to use...
Jainli, Strange, the effect is immediate (after clicking the OK button) for me. I also tried it on your cell72n series data (cell72n.034.tif) and unchecking...
Hi, John, Thank you very much and the problem solved. I did not realize I have to click red/green/blue twice to uncheck the color setting. This is a very cool...
I have a couple of questions: 1 – I am reconstructing data from various confocal image stacks which I have to piece together like a huge 3-D jigsaw puzzle. I...
Debbie, The acos DOMAIN error arises when the Boissonnat surfacing algorithm encounters invalid trace data, most likely traces that overlap within the section....
Hi, I have been using Reconstruct for some time now - but for the quantification of dendritic spine morphology. Briefly, I import confocal stack maximum...
Mike, One approach would be to copy the traces into a single section, merge them, and then get the area measurement from the trace list. This will work...
Hi all, We're running XP on a Mac through Parallels, and have had some strange problems, including corruption of a series file and some trace files not being...
Dear all, we moved to some large reconstructions, with many objects (250+). Although we apply strict naming conventions for the objects to make navigating the...
Thomas, I agree that it would be useful to allow more hierarchical organization of the Object List data and I'll add it to the TODO list, but there's no reason...
Dear Thomas, yes I would like that very much, too. I am as well reconstructing a huge amount of objects (~ 300), and while adding to a scene, it take me hours...
Reconstruct Users, I have posted a new version of Reconstruct (v1.0.9.0) at: http://tech.groups.yahoo.com/group/reconstruct_users/files/ This version adds the...
Dear John, Thank you for the answer and for the new release. I agree that for completely separate objects, your solution is the best (i.e: exporting one set of...
Reconstruct Users, The latest build (v1.0.9.1) available at: http://tech.groups.yahoo.com/group/reconstruct_users/files fixes the problem with horizontal lines...
Dear John, I was always wondering what the first point under "Wildfires" under "Series options" is. On my computer, I can´t read it, cause there is one line...
Jan, The first is line is "Stop growing when Hue", as in: stop wildfire region growing when the hue of a pixel differs by 50 from the hue of the starting...
Hi John, sorry I could not stay online enough... So here´s the screenshot I was talking about (see attachment). I am using a WinXP 3 GHz P4 computer with a...
Jan, Thanks for this information. I'll fix these problems with the Series Options dialog in the next version. Let me know if you find any other dialog text...
Reconstruct Users, I've posted a new version (v1.0.9.2) at: http://tech.groups.yahoo.com/group/reconstruct_users/files This version fixes a couple of bugs and...
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