hi all for those who are doing the 3d rendering with blender; there is a new modifier in the latest version of blender, which let you smooth objects with...
Hello all, I have a stack of images in reconstruct with several traces in each image. I try to figure out how the transformations and coordinates are coded in...
Dear John, Recently I started to measure volume and surface area of 3D objects. I found something strange. According to User's Manual, flat area =all open...
Hi, John, I am using recontruct to align to images with file size around 400MB each. When I tried to export the aligned image, reconstruct always reported "GDI...
Yoshi, The formula stated in the manual for Flat Area is correct: when trace is closed, section thickness is not used as multiplier. If you think Reconstruct...
Jianli, Did you try not drawing the traces on the exported images? I think that the GDI memory is only used during Export when traces are drawn. John....
Yoshi, Now I see what is confusing you. An "open" trace is one in which the last point is not connected to the first point, while a "Closed" trace starts and...
Hi, John, Thanks for your response. I tried to export images (>400MB) after I deleted all the traces and still failed. The error message varies though. This...
Dear Dr. Fiala, I am using Reconstruct to trace the dendritic arbor of neurons visualized using confocal microscopy. I have been tracing the processes using...
Alexis, You can display the Z-traces and object profiles together in the 3D scene, to see what you've traced already. But if you want to see where the Z-trace...
My name is Paula Silva and I am a PhD student. My work is been developed in Instituto Ciências Biomédicas Abel Salazar no Porto (Portugal). I am trying to...
Dear Nuno, I am in the end of my PhD and this the only experimental work that I have to do, as I don't have much time (I have to finish my thesis until the end...
Hello, I am a graduate student working on a project related to microtubules in neuronal processes. For this, I have to image individual pieces of microtubules...
Hello, I am a graduate student working on a project related to microtubules in neuronal processes. For this, I have to image individual pieces of microtubules...
Aditi, Yes, Reconstruct will be ideal for your project. You just need to make sure that you have thin enough sections and that you photograph your sections at...
Thank you John. Will the use of this software eliminate the need of old fashioned paper tracing of the section? Also, do I need any additional accessories to...
Aditi, I recommend scanning the negatives of your EM micrographs on a scanner with a transparency adaptor at about 1000dpi. These scanned images can be...
Thanks a lot John. I really appreciate your prompt replies. Aditi ... scanner with a transparency adaptor at about 1000dpi. These scanned images can be...
Reconstruct Users, I have posted a new build (version 1.0.9.8) at: http://tech.groups.yahoo.com/group/reconstruct_users/files/ This build boosts the menu...
Reconstruct Users, I just discovered that the program sometimes crashed when accessing the main menu while using the Domain Selection Tool (with a Domain ...
I am new to this group. I am expecting to generate about 60-75GB of data of approx. 4000-5000 serial section digital images of a structure. Will reconstruct...
Sanjeevan, 5000 sections, each a 15 Mb image, are no problem for the software because it basically just works with 2 sections at a time. Downsizing will not be...
Thanks a lot, John, for the tip. It would be great if you can kindly hint me the desirable graphic card requirements as well, as I am starting to assemble the...
Sanjeevan, The speed of rendering depends on the number of triangular surface patches there are in your 3D object. If your object was roughly a cylinder sliced...
hi please correct me, if i'm wrong, but recently, i tested the rendering speed on pc's and their graphic cards. the rendering speed doesn't depend on the speed...
German, Thanks for pointing this out: generating the 3D model (the triangles) certainly takes a lot longer than displaying the shaded surface in OpenGL...
Reconstruct Users, I've posted a new build v1.1.0.0 with only minor changes over v1.0.9.9. The new version can be downloaded with Help Manual and example.ser ...
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