My name is Paula Silva and I am a PhD student. My work is been developed in Instituto Ciências Biomédicas Abel Salazar no Porto (Portugal). I am trying to...
Dear Nuno, I am in the end of my PhD and this the only experimental work that I have to do, as I don't have much time (I have to finish my thesis until the end...
Hello, I am a graduate student working on a project related to microtubules in neuronal processes. For this, I have to image individual pieces of microtubules...
Hello, I am a graduate student working on a project related to microtubules in neuronal processes. For this, I have to image individual pieces of microtubules...
Aditi, Yes, Reconstruct will be ideal for your project. You just need to make sure that you have thin enough sections and that you photograph your sections at...
Thank you John. Will the use of this software eliminate the need of old fashioned paper tracing of the section? Also, do I need any additional accessories to...
Aditi, I recommend scanning the negatives of your EM micrographs on a scanner with a transparency adaptor at about 1000dpi. These scanned images can be...
Thanks a lot John. I really appreciate your prompt replies. Aditi ... scanner with a transparency adaptor at about 1000dpi. These scanned images can be...
Reconstruct Users, I have posted a new build (version 1.0.9.8) at: http://tech.groups.yahoo.com/group/reconstruct_users/files/ This build boosts the menu...
Reconstruct Users, I just discovered that the program sometimes crashed when accessing the main menu while using the Domain Selection Tool (with a Domain ...
I am new to this group. I am expecting to generate about 60-75GB of data of approx. 4000-5000 serial section digital images of a structure. Will reconstruct...
Sanjeevan, 5000 sections, each a 15 Mb image, are no problem for the software because it basically just works with 2 sections at a time. Downsizing will not be...
Thanks a lot, John, for the tip. It would be great if you can kindly hint me the desirable graphic card requirements as well, as I am starting to assemble the...
Sanjeevan, The speed of rendering depends on the number of triangular surface patches there are in your 3D object. If your object was roughly a cylinder sliced...
hi please correct me, if i'm wrong, but recently, i tested the rendering speed on pc's and their graphic cards. the rendering speed doesn't depend on the speed...
German, Thanks for pointing this out: generating the 3D model (the triangles) certainly takes a lot longer than displaying the shaded surface in OpenGL...
Reconstruct Users, I've posted a new build v1.1.0.0 with only minor changes over v1.0.9.9. The new version can be downloaded with Help Manual and example.ser ...
I have recently downloaded the software for Reconstruct, but I am having difficulty in opening any images. The Quick start chapter says that to test the...
Hi John and German, Thanks a lot for the information. I managed to get a pair of NVidia Quadro FX which claims about 145 million triangles/s each (that can be...
I am trying to burn (backup) my serial reconstructions and it burns to the CD successfully but when you try to open the file off of the CD the reconstruct...
Atticus, Depending on how your series was created and the version of the software used, your file references may be in absolute path mode rather than relative...
Hi All, I assumed since Reconstruct can open confocal images that it should be able to do the same for two photon images. My images are in TIF format but the...
How did your import your images? Kristen M. Harris, PhD Professor, Neurobiology Center for Learning and Memory NMS Room 5.306 The University of Texas at Austin...
I tried to import using the Reconstruct import tool and imported one at a time. These images are part of a Z-series but Reconstruct did not recognize the stack...
Try renaming the images so that they don't have the 'tiff' in the name, just numbers- -- In reconstruct_users@yahoogroups.com, Angelica Esquivel ... one at a...
Well, I should know the answer to the following question and probably do, but I'm going to see if anyone else has tried the following: We have a series of open...
All right -- I solved it myself -- It turned out that when I closed the contours for some of these open traces they became negative and hence unable to surface...
You probably should convert to a standard Tiff or Jpeg or other format as RECONSTRUCT regularly recognizes standard formats, but if Imaris were to have a...
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