Is there a way to list lengths of traces in the object table, or to get all traces across the series in a single table? I'm measuring mitochondria and...
Linnaea, One way would be to treat them as Z-lengths on a single section. Then the z-lengths table will give you the correct values all in one table -- like...
A new version is available at: http://synapses.bu.edu/tools/download.htm The users manual has been updated to reflect the new functionality. What's new? ...
John, I had a quick question. If I want to measure the amount of surface area (or contact area) between two objects that come into contact, do I simple draw...
Reconstruct users, Having trouble with z-trace notes or palette comments? A new build (v1.0.2.1) available from the File section of the Reconstruct users group...
Hi All, I've been using an older reconstruct program for about 4 months and have switched to this newer program. I have been trying to print my images with the...
Hi All, I am now trying to transfer my 3-D scene to our new program the 3ds max 6. The program takes vrml files but the wire frame of the traces does not come...
Kristen, To create a local copy of the users manual, download the manual.hlp file from the users group files and save it to the directory in which Reconstruct...
John, Turns out the file needs to be named "USERSMANUAL.HLP" in the RECONSTRUCT subdir -- otherwise only the first chapter opens from RECONSTRUCT and the rest...
Anusha, I think this may be a question of general interest so I'm posting it to the users group. The expansion of traces is caused by the simplify algorithm...
Reconstruct Users: I've posted a new build (v1.0.2.9) to the Files section of the users group. The main development in this build is the implementation of a ...
1. I have a sequence of PS 7 jpg's derived from em images. I have been able to link and align these images, but can see only the link points and not the...
You wrote: We played with this a bit. A setting of 150 gave the best automatic recons. however, the wildfire stopped at mitochondria, vesicles, and SER...
Reconstruct Users, A new version is available for download from http://synapses.bu.edu/tools/download.htm This latest version (v1.0.3.0) offers 2 enhancements:...
I hev built up a 3d sequence which has been running well producing a 3D scene of normal size until recently. The 3d image appears very small in its window....
Robert, The 3D Scene window has a bounding box that surrounds all the objects in the scene. The center of this box is the center of the scene rotation....
Robert wrote: However, I have already tried what you suggested. I still have a small image at top centre of the window. You mention object deletion: I did...
... From: "Sharron Fina" <sfina@...> To: reconstruct_users@yahoogroups.com Sent: Mon, 22 Nov 2004 15:49:36 -0400 Subject: Problem holes ...
Sharron Fina
sfina@...
Nov 22, 2004 8:31 pm
55
Sharron, Download and install the latest version from synapses.bu.edu (v.1.0.3.1). Create a new series, and select Import > Series... from the Series menu....
Mark, When you select a palette button, the button's trace name is copied to the default trace name. Subsequent tracing uses and updates the default trace name...
Dear Dr. Ovtscharoff, The Max error (pixels) parameter does not appear explicitly in Reconstruct, although perhaps it should? Currently, the simplification of...
Richard, ... 1. Occasionally when I try to generate a BMP file of a 3D scene, I get a file that is just garbage (attached file SingleProcess+Sublats)....
Hi, John, I am a new user for your software at Johns Hopkins U. I reconstruct a nice 3D image recently, however there was an error when I was trying to...
Hi Everyone, I was wondering if anyone has used either the reconstruct program or 3ds max 6 discreet to measure the area of your reconstructed cells. I am...
Angelica, I use Image J, a free downloadable program available from the NIH. Basically it is the PC version of NIH Image. http://rsb.info.nih.gov/ij/ It's...
Sharron Fina
sfina@...
Jan 3, 2005 10:55 pm
62
Angelica, Reconstruct has at least 4 ways to make distance measurements: 1) Use the line or open point-by-point tools within a section, and then list the...
Ju, You wrote: 1. It seems that when I choose the Object list, it takes very long to read the list, even if there are only a few objects. In addition, I feel...
Why not just use the z-tool or a line to make this measurement? We use it routinely to determin mitochondrial diameters -- just measure the greatest diameter....
We are trying to use reconstruct on histological slide section of different pathological tissues. So for an entire histological section I need to add a stained...
Storey, Yes, I see that adding such a feature to Reconstruct would be beneficial. I'll put it on the TODO list. IGL Trace has such a function called Trace...
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