Hi All, I assumed since Reconstruct can open confocal images that it should be able to do the same for two photon images. My images are in TIF format but the...
How did your import your images? Kristen M. Harris, PhD Professor, Neurobiology Center for Learning and Memory NMS Room 5.306 The University of Texas at Austin...
I tried to import using the Reconstruct import tool and imported one at a time. These images are part of a Z-series but Reconstruct did not recognize the stack...
Try renaming the images so that they don't have the 'tiff' in the name, just numbers- -- In reconstruct_users@yahoogroups.com, Angelica Esquivel ... one at a...
Well, I should know the answer to the following question and probably do, but I'm going to see if anyone else has tried the following: We have a series of open...
All right -- I solved it myself -- It turned out that when I closed the contours for some of these open traces they became negative and hence unable to surface...
You probably should convert to a standard Tiff or Jpeg or other format as RECONSTRUCT regularly recognizes standard formats, but if Imaris were to have a...
They might be "proprietary" TIFF or incorrectly written TIFF images. Try "washing" them through Photoshop which in my experience does a pretty good job...
Hello, When I tried to open a folder I have been working on for a while the application said 'insufficient memory to complete operation' and then 'abnormal...
I'm not sure what might have happened. I haven't experienced the problem you are describing, so I can't be sure the following will correct the problem but...
Dear all, It might be the after-effects of incompletely metabolized Christmas champagne but I have registered a whole serie of EM sections with a 10x error for...
Okay - here's what to do. I just tried it to remind myself. Go to the calibration section. Select all the traces, Set to the size you want. Then open the...
Thank you for the answer, I am still having trouble and I not sure whether I am properly following your instructions. a) I went to "Section/List sections" b)...
How do you know what the pixel size is supposed to be? If you did a proper calibration, it should be correct. I'm a bit mystified, but I'll be interested to...
Hello, Initially, I usually ignore pixel size when importing the sections and I then immediately do a calibration before anything else, selecting the option...
Hello everybody, can anyone tell me how I can measure the distance of individual immunogold particles to a reference point by using reconstruct? Thank you very...
Hi everybody, Following John's request, I just added an alternative link to download Reconstruct, the quick start guide, and the manual in the "Links" section...
Thanks Basilis, If anyone has trouble, you may also download the last build from our website Synapse Web under tools located at synapses.clm.utexas.edu Kristen...
Hello, This email message is a notification to let you know that a file has been uploaded to the Files area of the reconstruct_users group. File :...
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Feb 16, 2008 4:09 pm
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Hi everyone, I'm a new Reconstruct user. The lab where I was working at used the Neurolucida system with a motorised stage (Z- Axis) to capture image stacks...
If your images are already aligned, when you import them into Reconstruct they will remain aligned. Just lock the section and you won't 'unalign' them. You can...
Hello, This is a 'calibration' question. I reconstructed structures from physical sections, at first without calibrating. I finally decided to analyse my...
Hi, Is it possible to shift (i.e. translate left/right etc) whole image stacks once they've been imported into Reconstruct? And if so, is it possible to shift...
Hello, I need to rename a lot of objects in reconstruct, like dendrite01_Neuron02 to neur02_dend01, dendrite02_Neuron02 to neur02_dend02 .... I can do this...
Doesn't look like you have a standard pattern to rename. The easiest what to do this is with rename in the object list, then all the traces associated with the...
Hello, I solved my problem with a keystroke/mouse movement logger (Smack 1.06). It records all keystrokes including Backspace, Ctrl-C, arrows,.... It even...
Thanks Thomas - sounds very clever, we are in the process of updating the RECONSTRUCT manual with all the FAQ's from the users group and cool things like this....
Thomas - could you share your actual macro so that we can see how you interfaced to RECONSTRUCT to perform this function? Thanks, Kristen M. Harris, PhD ...
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