Hi, Is it possible to shift (i.e. translate left/right etc) whole image stacks once they've been imported into Reconstruct? And if so, is it possible to shift...
Hello, I need to rename a lot of objects in reconstruct, like dendrite01_Neuron02 to neur02_dend01, dendrite02_Neuron02 to neur02_dend02 .... I can do this...
Doesn't look like you have a standard pattern to rename. The easiest what to do this is with rename in the object list, then all the traces associated with the...
Hello, I solved my problem with a keystroke/mouse movement logger (Smack 1.06). It records all keystrokes including Backspace, Ctrl-C, arrows,.... It even...
Thanks Thomas - sounds very clever, we are in the process of updating the RECONSTRUCT manual with all the FAQ's from the users group and cool things like this....
Thomas - could you share your actual macro so that we can see how you interfaced to RECONSTRUCT to perform this function? Thanks, Kristen M. Harris, PhD ...
Dear Dr. Harris, The actual macro depends on the position of the windows on the screen (it records mouse position) so would be of little use. Here is instead ...
I should add that the procedure probably work with many of the free/shareware/commercial macro recorders available. I tried Smack because it was the first on a...
Thanks Thomas. I have some mac-savvy and macro savvy folks in the lab who will appreciate this. Best, Kristen Kristen M. Harris, PhD Professor, Neurobiology ...
I've just started using Reconstruct and made the mistake of not tracing all my traces in the same direction (clockwise). Is there a way to select a trace and...
Dear Mat, In the Trace menu, select "Reverse". You should also select "Automatically simplify new closed traces" in Serie/Options/Autotracing which takes care...
Hi: One way that you can verify that a trace has a clockwise or counterclockwise (i.e. "reversed") orientation is by checking the area measurement in the...
Hi, I'm trying to obtain the value of the volume of some spines. I understand that the volume is computed using the Cavalieri formula, but my question is where...
In the Object list - first go to series options>List>Objects>click on the values you want to display - in this case volume. Kristen M. Harris, PhD Professor,...
Hi Rosa, Just open the "Series Options" with "Strg"+"o" (or via GUI menu "Series/Options"), there you go to the "Lists"-Tab and activate the approbiate...
Hi again, I have another very basic question, this time about the areas. What is the difference between flat area and surface area?, I noticed that the values...
I suggest you read the manual carefully about this one. It explains it well. Kristen M. Harris, PhD Professor, Neurobiology Fellow, Center for Learning and...
Hello, I was wondering if it is possible to export a 3D scene so that it could be presented as an avi file or some other file that can play in microsoft power...
Yes - If you export as a VMRL file, you should be able to import it into an AVI maker - though what I usually do is have the movie running in a VMRL viewer...
Freez Screen Video Capture at: http://www.smallvideosoft.com/ With this you can capture on a avi file whathever you do on your desktop. You can play the 3D...
Hello, everyone, I am trying to find a way to export the x,y,z coordinate of the objects. Now I have around 500 objects in a stack, each represented by a...
... I have been using two methods successfuuly for the last 2 years or so. 1. To view a VRML file: import the cortona plugin into p/point. The plugin can...
Hey, everyone. I am reconstructing immunolabelled spines and PSDs, but I can't figure out how to place icons on the immunogold particles and NOT have ...
Hi Dan, Here is what I do. - Go to Serie/Serie Option/3D => choose Boissonnat surface - Go to Object / List Objects => select the PSD and spine - Go to Scene /...
Thanks, Thomas and Rick. I am pursuing Thomas's solution, and it works well. The only problem is that multiple particles on single sections are considered one...
I think I figured it out. When you place objects with the stamp tool onto the particles, you need to delete the $ from the name in the trace attributes. Thanks...
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