Dear all, Is it possible to leave one edge of a 3D reconstruction open so that you can see inside your object? I know you can make your reconstructions...
Yes - go to Series-Options-3D and unclick the checkmark in the 'generate top and bottom faces' at the bottom under Boissonnat Surfaces. --If you unclick either...
Is there any solution to avoid the mislocalization and size reduction of objects in the 3D scene after Boissonnat surfacing resulting from the process that the...
Boissannatt surfacing as I understand it requires that the traces on adjacent sections have overlap. You might want to look in the blend mode to see whether...
Hi Marcus You can also zoom in the 3D scene window until whatever surface is at the front is zoomed through, and then you can see inside. This option lets you...
Let me refine the problem, because I was not correct in all sense: Not both endings of the objects are reduced when Boissonnat surface is applied. Instead, the...
Hi Kristof, I'm forwarding this message to Larry Lindsey who is currently 'refactoring' RECONSTRUCTTM so that we can add new features in the future. He is...
Thanks, Kristen. You are an unbelievable scientist. And, for you to continue your commitment to Reconstruct AND continue its development, I believe you are...
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May 31, 2009 2:23 am
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You make me blush! From: reconstruct_users@yahoogroups.com [mailto:reconstruct_users@yahoogroups.com] On Behalf Of Dan Nicholson Sent: Saturday, May 30, 2009...
Hi there I am basically wondering how exactly wildfire works. I notice that at high magnification, pixels are visable and the subtly varying shades of (in my...
Hi all, I would be very thankful if someone could tell me how I can add immunogold particles to my reconstructed dendrites? I tried several options but always...
Hi, Lax. Please the message below from Thomas, as well as the next message from Kristen. Let me know if this works for you. Dan ... Subject: [Reconstruct] Re:...
The second tip from the brilliant, inimitable, and indefatigable Kristen Harris. Dan ... Subject: RE: [Reconstruct] Immunogold particles Date: Fri, 31 Oct...
Here's my method. 1. First reconstruct the structure that you need to display in 3D using the conventional method. 2. One gold particle should be treated as...
I was not fast enough to see replies from Dan. Anyway, I find that naming the object is very important especially if you have more than hundreds of gold...
Yes, however, I caution you that the best thing to do is to first stamp all of your objects with the same name, not using the + to count, because if you then...
Thanks to all those who replied. I really appreciate it. The tips given here were useful to me. One more question: I would like to measure the distance of...
Dear all, To measure the distance between each particle and the center of the PSD, you will first have to define where, in 3D, that center is. This is...
Hi, I have difficulties making a hole through an object. The manual says you can use counterclockwise traces to define holes, but I do not succeed. I need it...
Hi, Usually we give different traces its own label through a name. When I have a high number of similar structures (like unmyelinated axons or mitochondria)...
Hello Reconstruct Users, My name is Larry Lindsey, and I have recently started in the Kristen Harris Lab as the main developer for Reconstruct. Soon, I intend...
hi larry I am happy to hear, that something is going on with reconstruct. I have a small thing, which could be improved: There is a field in the menu...
Hi Larry, I have been using Reconstruct for 2-3 years now and have always been mystified by the wildfire routines. I have generally found it quicker to draw...
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