Hi there I am basically wondering how exactly wildfire works. I notice that at high magnification, pixels are visable and the subtly varying shades of (in my...
Hi all, I would be very thankful if someone could tell me how I can add immunogold particles to my reconstructed dendrites? I tried several options but always...
Hi, Lax. Please the message below from Thomas, as well as the next message from Kristen. Let me know if this works for you. Dan ... Subject: [Reconstruct] Re:...
The second tip from the brilliant, inimitable, and indefatigable Kristen Harris. Dan ... Subject: RE: [Reconstruct] Immunogold particles Date: Fri, 31 Oct...
Here's my method. 1. First reconstruct the structure that you need to display in 3D using the conventional method. 2. One gold particle should be treated as...
I was not fast enough to see replies from Dan. Anyway, I find that naming the object is very important especially if you have more than hundreds of gold...
Yes, however, I caution you that the best thing to do is to first stamp all of your objects with the same name, not using the + to count, because if you then...
Thanks to all those who replied. I really appreciate it. The tips given here were useful to me. One more question: I would like to measure the distance of...
Dear all, To measure the distance between each particle and the center of the PSD, you will first have to define where, in 3D, that center is. This is...
Hi, I have difficulties making a hole through an object. The manual says you can use counterclockwise traces to define holes, but I do not succeed. I need it...
Hi, Usually we give different traces its own label through a name. When I have a high number of similar structures (like unmyelinated axons or mitochondria)...
Hello Reconstruct Users, My name is Larry Lindsey, and I have recently started in the Kristen Harris Lab as the main developer for Reconstruct. Soon, I intend...
hi larry I am happy to hear, that something is going on with reconstruct. I have a small thing, which could be improved: There is a field in the menu...
Hi Larry, I have been using Reconstruct for 2-3 years now and have always been mystified by the wildfire routines. I have generally found it quicker to draw...
Hi Larry, Totally agree with Robert, the logic behind the parameters of the wildfire tool has been kind of a mystery to me, and thus i rarely use it. A more...
Wildfire is really not very user-friendly for TEM - I also do not use it. It is better for cameral lucida tracings or other objects that have definitive...
Dear all, As said previously, Wildfire can work wonder for Camera Lucida or fluorescence confocal microscopy but TEM are too "messy" (mitochondria, microtubule...
Hi all, I'm working on my master thesis in microbiology at the University of Innsbruck. I was searching for a way to reconstruct serial sections when I came...
Dear Larry, I am also able to extend the improvement list a little bit :) concerning the alignment function of Reconstruct. It would be particulary useful to...
Hi Larry, Although Kristen told me he would forward my problem and suggestion to you regarding Boissonnat surface generation, I repeate it to you by copying my...
Dear Larry, The other problem I've had with Boissonnat surfacing is the following: I've got eg. an axonterminal with an amorf surface, and I've got some...
Hi Kristof, Kristen Harris is a 'she'.. J Thanks Kristen From: reconstruct_users@yahoogroups.com [mailto:reconstruct_users@yahoogroups.com] On Behalf Of...
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