Reconstruct Users: I've posted a new build (v1.0.2.9) to the Files section of the users group. The main development in this build is the implementation of a ...
1. I have a sequence of PS 7 jpg's derived from em images. I have been able to link and align these images, but can see only the link points and not the...
You wrote: We played with this a bit. A setting of 150 gave the best automatic recons. however, the wildfire stopped at mitochondria, vesicles, and SER...
Reconstruct Users, A new version is available for download from http://synapses.bu.edu/tools/download.htm This latest version (v1.0.3.0) offers 2 enhancements:...
I hev built up a 3d sequence which has been running well producing a 3D scene of normal size until recently. The 3d image appears very small in its window....
Robert, The 3D Scene window has a bounding box that surrounds all the objects in the scene. The center of this box is the center of the scene rotation....
Robert wrote: However, I have already tried what you suggested. I still have a small image at top centre of the window. You mention object deletion: I did...
... From: "Sharron Fina" <sfina@...> To: reconstruct_users@yahoogroups.com Sent: Mon, 22 Nov 2004 15:49:36 -0400 Subject: Problem holes ...
Sharron Fina
sfina@...
Nov 22, 2004 8:31 pm
55
Sharron, Download and install the latest version from synapses.bu.edu (v.1.0.3.1). Create a new series, and select Import > Series... from the Series menu....
Mark, When you select a palette button, the button's trace name is copied to the default trace name. Subsequent tracing uses and updates the default trace name...
Dear Dr. Ovtscharoff, The Max error (pixels) parameter does not appear explicitly in Reconstruct, although perhaps it should? Currently, the simplification of...
Richard, ... 1. Occasionally when I try to generate a BMP file of a 3D scene, I get a file that is just garbage (attached file SingleProcess+Sublats)....
Hi, John, I am a new user for your software at Johns Hopkins U. I reconstruct a nice 3D image recently, however there was an error when I was trying to...
Hi Everyone, I was wondering if anyone has used either the reconstruct program or 3ds max 6 discreet to measure the area of your reconstructed cells. I am...
Angelica, I use Image J, a free downloadable program available from the NIH. Basically it is the PC version of NIH Image. http://rsb.info.nih.gov/ij/ It's...
Sharron Fina
sfina@...
Jan 3, 2005 10:55 pm
62
Angelica, Reconstruct has at least 4 ways to make distance measurements: 1) Use the line or open point-by-point tools within a section, and then list the...
Ju, You wrote: 1. It seems that when I choose the Object list, it takes very long to read the list, even if there are only a few objects. In addition, I feel...
Why not just use the z-tool or a line to make this measurement? We use it routinely to determin mitochondrial diameters -- just measure the greatest diameter....
We are trying to use reconstruct on histological slide section of different pathological tissues. So for an entire histological section I need to add a stained...
Storey, Yes, I see that adding such a feature to Reconstruct would be beneficial. I'll put it on the TODO list. IGL Trace has such a function called Trace...
Xiping wrote: Reconstruct seems not to save changes after the changes are done. For example, I made some of the object "trasparent " in Scene, and it went back...
Hi everyone, I've just started using reconstruct and am having a few teething problems. When I try to create a 3d image from objects in the object list...
Hello, This email message is a notification to let you know that a file has been uploaded to the Files area of the reconstruct_users group. File :...
reconstruct_users@yah...
Jan 25, 2005 1:34 pm
70
... I guess it depends on how you would use such an organization. If you use it to limit the view of the data, e.g. show me only the objects associated with...
Reconstruct Users, An update is available from the files section of the users group (http://groups.yahoo.com/group/reconstruct_users/). The update has a few...
Hi, I've been tracing for only a month or so now (newbie) and have encountered some difficulty with donut shaped traces. I use the wildfire tool and, just as...
Michael, To define an interior boundary of an object you'll need to use a Reverse trace. This is done by drawing a normal trace and then using the Reverse item...
Dear John, I have a couple issues that I need help with... First Issue: I have come across a problem in renaming traces in the z- trace list. When I have two...
... [the] data to excel spreadsheets a lot more efficient than doing it from the trace list. Anusha, Try using the Series>Export>Trace Lists... function to get...
I'm still having problems with the simplify command and my images. First, even though I turn off simplify when I create the .ser it seems to be turned on again...
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