Currently im working with NPY stained axon reconstruction. In one location, the axon is almost parallel with the cut of the thin thin section. Thus, there...
Kevin, While it's not possible to draw on selected domains, ie. to create correspondence points, it is possible to align using points draw on the section just...
It appears someone has been using the Reconstruct Users group to send computer viruses. Please do not open any attachments addressed to the users group. If...
Jon, It is also possible that when hitting Ctrl-D you hit the F key also. In version 1.0.4.5 this invokes the Wildfire autotracing operation. I'll try to fix...
I will be out of the office starting Fri 05/13/2005 and will not return until Tue 05/24/2005. beste, ik ben er niet tot dinsdag 24 mei 2005. I'm not at the...
Dr. Fiala, I also have similar problems with Ctrl+D. The program acts like I hit the F key for wildfire autotracing. When I select an existing trace, hitting...
Reconstruct Users, The latest update to Reconstruct (v1.0.4.6) fixes the Ctrl-D problem that existed in version 1.0.4.5. In version 1.0.4.6 the pencil can no...
Dr. Kubota, When you open the Object List the program reads all of the sections to see what traces have been created and to compute the values for the list...
Reconstruct Users, I've posted a new version (v1.0.4.7) with enhanced Wildfire tool functionality: http://groups.yahoo.com/group/reconstruct_users/files/ ...
Dear Dr. Fiala, I do not know how difficult it can be, but I would like to suggest if it would be possible to get more user-defineable traces in the trace...
Dear Dr. Fiala, My procedure is to digitalize all components and measurements of each spine (say spine 239) because I need all ten traces for each spine (239...
Storey, Thanks for the sample image. I've found and fixed the bug, and will post an update to the users group this afternoon. The new version will include a...
Reconstruct Users, A new version is available at: http://groups.yahoo.com/group/reconstruct_users/files/ Version 1.0.4.9 changes the keyboard interface for...
Dear all, I want to know how to put 2 picture together together to form one picture? I mean I take 2 pictures from the section(2/3 of section for each picture)...
Bin Ma, Reconstruct is useful for any serial sections, including those digitized from light microscopy. I have used it for conventionally stained light...
Reconstruct Users, The latest version of Reconstruct (v1.0.5.0) is available from: http://synapses.bu.edu/tools/download.htm along with documentation updated...
Hi John, Thanks for the update to Reconstruct. Great program. Since you've updated the documentation, I thought I'd download local copies. Everything opened...
Deanna, To reorder some sections in your series use the Numbering... item from the Modfiy menu of the Section List. First highlight the sections you want to...
Dear Dr. Fiala, It is me again... It is just to say that quite often, after tracing a line in the sections, if I open the 3D view, this line appears in the...
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Jon, Try version 1.0.5.1 (http://groups.yahoo.com/group/reconstruct_users/files/) and see if this fixed your problem. In v1.0.5.1 a scene Reset should always...
Dear John, me again, I thougth the bug was solved since it did not happen again for a while, but suddenly.... it appeared again. At least I have more detailed...
Jon, Are you try to generate a Boissonnat surface for an open polyline? This will not work because Boissonnat's algorithm is only for closed traces, not open...
Jon, OK, see if I've really fixed it now in v1.0.5.2 (http://groups.yahoo.com/group/reconstruct_users/files/)... Also, take a look at the modified Object List...
Currently im still aligning serial EM sections, but we're now reconstructing dendritic areas where the sections are about 15-25 images montaged. My question...
Steve, Sorry to hear version 1.0.5.2 is unstable. Perhaps I broke something related to the creation of Z-traces. I'll check on it. Any details on exactly WHEN...
Bin Ma, A semiautomated segmentation that seems to work well for confocal is the Wildfire Tool (http://synapses.bu.edu/tools/chapter8.pdf). For a description...
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